1.21.1.1: iodotyrosine deiodinase
This is an abbreviated version!
For detailed information about iodotyrosine deiodinase, go to the full flat file.
Word Map on EC 1.21.1.1
-
1.21.1.1
-
thyroid
-
iodide
-
deiodination
-
hypothyroidism
-
diiodotyrosine
-
thyroglobulin
-
dehalogenation
-
halotyrosines
-
iodothyronine
-
nitroreductase
-
goiter
-
bromo
-
chlorotyrosine
-
organification
-
diagnostics
-
synthesis
-
medicine
- 1.21.1.1
- thyroid
- iodide
-
deiodination
- hypothyroidism
- diiodotyrosine
- thyroglobulin
-
dehalogenation
-
halotyrosines
-
iodothyronine
- nitroreductase
- goiter
-
bromo
-
chlorotyrosine
-
organification
- diagnostics
- synthesis
- medicine
Reaction
Synonyms
DEHAL1, Dehal1 protein, DEHAL1B, EC 1.22.1.1, iodotyrosine dehalogenase 1, iodotyrosine deiodinase, IYD, lyd, NADH oxidase/flavin reductase, SUP-18, SUP-18 IYD, TDH
ECTree
1.21.1.1 iodotyrosine deiodinaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.21.1.1 - iodotyrosine deiodinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-iodophenol + NADP+ + iodide
?
2-iodophenol binds very weakly to the enzyme and is dehalogenated with a catalytic efficiency that is more than 4 orders of magnitude lower than that for 3-iodo-L-tyrosine
-
-
?
L-tyrosine + 2 NADP+ + 2 bromide
3,5-dibromo-L-tyrosine + 2 NADPH + 2 H+
-
halide elimination does not appear to limit reactions of bromo- and iodotyrosine since both fully oxidize the reduced enzyme with nearly equivalent second-order rate constants despite the differing strength of their carbon-halogen bonds
-
-
r
L-tyrosine + 2 NADP+ + 2 chloride
3,5-dichloro-L-tyrosine + 2 NADPH + 2 H+
-
chlorotyrosine reacts with the reduced enzyme approximately 20fold more slowly than bromo- and iodotyrosine and reveals a spectral intermediate that forms at approximately the same rate as the bromo- and iodotyrosine reactions
-
-
r
3,5-diiodo-L-tyrosine + dithionite
3-iodo-L-tyrosine + ? + I-
-
solubilized enzyme preparations are active with dithionite, but not with NADPH. Particle-bound enzyme uses both dithionite and NADPH. At concentrations of substrate below 1 microM, 3,5-diiodo-L-tyrosine is more rapidly deiodinated than 3-iodo-L-tyrosine, which is reversed at concentrations greater than 5 microM
-
-
?
3,5-diiodo-L-tyrosine + NADPH + H+
3-iodo-L-tyrosine + NADP+ + I-
-
solubilized enzyme preparations are active with dithionite, but not with NADPH. Particle-bound enzyme uses both dithionite and NADPH. At concentrations of substrate below 1 microM, 3,5-diiodo-L-tyrosine is more rapidly deiodinated than 3-iodo-L-tyrosine, which is reversed at concentrations greater than 5 microM
-
-
?
3,5-diiodo-L-tyrosine + NADPH + H+
3-iodo-L-tyrosine + NADP+ + I-
-
-
-
?
3,5-diiodo-L-tyrosine + NADPH + H+
3-iodo-L-tyrosine + NADP+ + I-
-
-
-
-
?
3,5-diiodo-L-tyrosine + NADPH + H+
3-iodo-L-tyrosine + NADP+ + I-
substrate 3-iodo-L-tyrosine is preferred over 3,5-diiodotyrosine
-
-
?
3-bromo-L-tyrosine + NADP+ + bromide
3,5-dibromo-L-tyrosine + NADPH + H+
-
-
-
?
3-bromo-L-tyrosine + NADP+ + bromide
3,5-dibromo-L-tyrosine + NADPH + H+
-
-
-
?
3-chloro-L-tyrosine + NADP+ + chloride
3,5-dichloro-L-tyrosine + NADPH + H+
-
-
-
?
3-chloro-L-tyrosine + NADP+ + chloride
3,5-dichloro-L-tyrosine + NADPH + H+
low activity
-
-
?
3-iodo-L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
-
-
-
?
3-iodo-L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
-
-
-
?
3-iodo-L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
-
-
-
?
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
-
-
-
?
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
-
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
stepwise single electron transfer involving FMN, ferredoxin, and NADPH, overview. Ability of the substrate to provide multiple interactions with the isoalloxazine system ofFMN that are usually provided by protein side chains. Ligand binding acts to template the active site geometry and significantly stabilize the one-electron-reduced semiquinone form of FMN
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
via mono-iodotyrosine
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
halide elimination does not appear to limit reactions of bromo- and iodotyrosine since both fully oxidize the reduced enzyme with nearly equivalent second-order rate constants despite the differing strength of their carbon-halogen bonds
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
via mono-iodotyrosine, the reaction might involve an additional ferredoxin reductase
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
-
-
-
r
L-tyrosine + 2 NADP+ + 2 iodide
3,5-diiodo-L-tyrosine + 2 NADPH + 2 H+
-
-
-
-
r
L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
overall reaction
-
-
?
L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
overall reaction
-
-
?
L-tyrosine + NADP+ + iodide
3,5-diiodo-L-tyrosine + NADPH + H+
overall reaction
-
-
?
L-tyrosine + NADP+ + iodide
3-iodo-L-tyrosine + NADPH + H+
-
-
-
?
additional information
?
-
no activity with 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
-
no activity with 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
a synergy between substrate selectivity and catalytic activity is created by the enzyme. No activity with the substrate analog 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
-
a synergy between substrate selectivity and catalytic activity is created by the enzyme. No activity with the substrate analog 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
analytical detection method for iodotyrosine, overview
-
-
?
additional information
?
-
-
analytical detection method for iodotyrosine, overview
-
-
?
additional information
?
-
-
fluorotyrosine is an inert substrate analogue
-
-
?
additional information
?
-
the enzyme can act as a general dehalogenase, promoting reductive dehalogenation of 3-bromo- and 3-chloro-L-tyrosine, though not 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
-
the enzyme can act as a general dehalogenase, promoting reductive dehalogenation of 3-bromo- and 3-chloro-L-tyrosine, though not 3-fluoro-L-tyrosine
-
-
?
additional information
?
-
-
while L-iodotyrosines are almost completely dehalogenated, D-iodotyrosines, alpha-methyl-DL-iodotyrosines and 3,5-diiodo-4-hydroxyphenyl-DL-lactic acid are poor substrates for the deiodinase. No substrates are 3,5-diiodo-4-hydroxyphenyl-alpha-guanidyl propionic acid, 3,5-diiodo-4-hydroxyphenyl propionic acid, 3,5-diiodotyramine, 3-iodo-5-nitro-L-tyrosine and 3-iodo-L-phenylalanine
-
-
?
