1.20.4.4: arsenate reductase (thioredoxin)
This is an abbreviated version!
For detailed information about arsenate reductase (thioredoxin), go to the full flat file.
Reaction
Synonyms
ARS, ArsC, ArsC1, ArsC1', ArsC3, arsenate reductase, Bs_ArsC, pI258 arsenate reductase, plasmid pI258 arsenate reductase, Sa_ArsC, Strop634, thioredoxin-arsenate reductase complex, thioredoxin-coupled arsenate reductase, Trx-coupled arsenate reductase
ECTree
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Cloned
Cloned on EC 1.20.4.4 - arsenate reductase (thioredoxin)
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C10S/C15A/C89L mutant and C10S/C15A/C82S mutant expressed in Escherichia coli
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli. Wild-type enzyme and the Cys mutants (C15A, C10A, C82A, C82S, C89A, C10SC15S, C10SC15A) are expressed in Escherichia coli. Wild-type enzyme, mutant enzyme C15A, mutant enzyme C10A, mutant enzyme C82S, mutant enzyme C89A, and mutant enzyme C10SC15A are expressed soluble and with high yields. Mutant enzyme C82A is found in inclusion bodies, and the double mutant C10S/C15S is not expressed
overproduced in Escherichia coli K38(pGP1-2)