1.2.7.4: anaerobic carbon-monoxide dehydrogenase
This is an abbreviated version!
For detailed information about anaerobic carbon-monoxide dehydrogenase, go to the full flat file.
Word Map on EC 1.2.7.4
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1.2.7.4
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co2
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codhs
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nickel
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acetogenic
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thermoaceticum
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rubrum
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rhodospirillum
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methanosarcina
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carboxydothermus
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hydrogenoformans
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carboxidovorans
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c-clusters
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oligotropha
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wood-ljungdahl
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co-dependent
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methanogenesis
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carboxydotrophic
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co-oxidizing
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hydrogenases
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barkeri
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sulfate-reducing
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nickel-dependent
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acetobacterium
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nifes
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molybdenum-containing
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hydrogenogenic
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acetogenesis
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nickel-containing
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syngas
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methyl-coenzyme
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moorella
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square-planar
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homoacetogenic
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overpotential
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acetate-grown
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fe-containing
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electrocatalysts
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analysis
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hydrogenophaga
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methyltetrahydrofolate
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cubane-type
- 1.2.7.4
- co2
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codhs
- nickel
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acetogenic
- thermoaceticum
- rubrum
- rhodospirillum
- methanosarcina
- carboxydothermus
- hydrogenoformans
- carboxidovorans
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c-clusters
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oligotropha
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wood-ljungdahl
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co-dependent
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methanogenesis
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carboxydotrophic
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co-oxidizing
- hydrogenases
- barkeri
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sulfate-reducing
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nickel-dependent
- acetobacterium
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nifes
-
molybdenum-containing
-
hydrogenogenic
-
acetogenesis
-
nickel-containing
-
syngas
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methyl-coenzyme
- moorella
-
square-planar
-
homoacetogenic
-
overpotential
-
acetate-grown
-
fe-containing
-
electrocatalysts
- analysis
-
hydrogenophaga
- methyltetrahydrofolate
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cubane-type
Reaction
Synonyms
acetyl coenzyme A synthase/carbon monoxide dehydrogenase, acetyl-CoA synthase/carbon monoxide dehydrogenase, ACS/CODH, Carbon dioxide/carbon monoxide oxidoreductase, Carbon monoxide dehydrogenase, carbon monoxide dehydrogenase-corrinoid enzyme complex, carbon monoxide dehydrogenase/acetyl-CoA synthase, Carbon monoxide oxidoreductase, Cdh, CO dehydrogenase, CO dehydrogenase complex, CO dehydrogenase complex II, CO dehydrogenase II, CO dehydrogenase/acetyl-CoA synthase, Co-DG, CO-DH, CODH, CODH II, CODH-II, CODH/ACS, CODHACS, CODHase, CODHII, coos, Dehydrogenase, carbon monoxide, EC 1.2.99.2, monoxide dehydrogenase/acetyl-CoA synthase, Ni,Fe-CODH, Ni,Fe-dependent carbon monoxide dehydrogenase, Ni-CODH, Ni-containing carbon monoxide dehydrogenase, Ni-Fe carbon monoxide dehydrogenase II
ECTree
1.2.7.4 anaerobic carbon-monoxide dehydrogenaseAdvanced search results
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results (Metals Ions
Metals Ions on EC 1.2.7.4 - anaerobic carbon-monoxide dehydrogenase
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METALS and IONS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
4Fe-4S cluster
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core extrusion experiments indicate 6 [4Fe-4S] clusters per tetramer, and electron paramagnetic resonance spectroscopy detects at least one of these clusters, in the reduced form
copper
Cu2+
Fe-S center
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the multifunctional enzyme complex contains iron-sulfur centers
Fe2+
Iron
Molybdenum
Ni2+
Nickel
Zinc
[2Fe-2S]-center
the small subunit contains two [2Fe-2S] centers
additional information
copper
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dinuclear heterometal [CuSMo(=O)OH] cluster in the active site
copper
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synthesis and characterization of dinuclear Mo-Cu complexes relevant to the active site of MoCu-enzyme by X-ray diffraction studies and by reactivity
Cu2+
the native enzyme contains copper in the active site
Fe2+
Ni-, Fe-, and S-containing metal center called cluster C
Fe2+
CODHII contains iron, the active site of CODH contains a [NiFe4S4OHx] cluster known as C-cluster
Iron
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the alphabeta dimer contains approximately 9-11 mol of iron and 12-14 mol of acid-labile sulfur per mol of dimer
Iron
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0.58, 0.67 and 1.96 atoms of iron per mol of large, medium- and small subunit, two iron-sulfur centers are associated with the small subunit
Iron
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contains 25 mol of Fe2+ and 20 mol of S2- per mol of tetramer, the enzyme contains 2 (4Fe-4S)+ clusters
Iron
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enzyme contains 3 iron containing centers: center A contains nickel and iron and is suggested to be the acetyl-CoA cleavage site, center B is a [4Fe-4S]2+/1+ center, center C is a fast relaxing center
Iron
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CO dehydrogenase complex consists of a two-subunit nickel/iron-sulfur component and the two-subunit factor III-containing corrinoid/iron-sulfur (Co/Fe-S) component
Iron
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the NiFe complex is required for catalyzing the exchange reaction and the acetyl-CoA synthase reaction
Iron
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CO oxidation occurs at Ni- and FeS containing center C, electrons are transferred from cluster C via center B to external electron acceptors
Iron
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the alphabeta dimer contains approximately 9-11 mol of iron and 12-14 mol of acid-labile sulfur per mol of dimer
Iron
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contains 11 mol of iron and 14 mol of acid-labile sulfur per mol of alphabeta dimer
Iron
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enzyme utilizes three types of Fe-S clusters: a [Ni4Fe5S] C-cluster catalyzing the CO oxidation, and 2 distinct [4Fe4S] electron-transfer sites called clusters B and D
Iron
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the enzyme contains two metal centers: a Ni-X-[4Fe-4S]2+/1+ cluster, i.e. C-center, that serves as the CO-oxidation site and a standard [Fe4S4]2+/1+ cluster, i.e. B-center, that mediates electron flow from the C-center to the external electron acceptor
Molybdenum
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molybdopterin-cytosine dinucleotide cofactor is composed of a molybdenum ion with 2 oxo- and 1 hydroxoligand complexed by the enedithiolene group of molybdopterin
Molybdenum
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dinuclear heterometal [CuSMo(=O)OH] cluster in the active site
Molybdenum
contains molybdenum, essential for activity
Molybdenum
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synthesis and characterization of dinuclear Mo-Cu complexes relevant to the active site of MoCu-enzyme by X-ray diffraction studies and by reactivity
Ni2+
Ni-, Fe-, and S-containing metal center called cluster C
Ni2+
CODHII contains nickel, the active site of CODH contains a [NiFe4S4OHx] cluster known as C-cluster
Ni2+
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required. Nickel is inserted into CODH without the need to express the native Ni insertase protein
Ni2+
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Ni is tightly bound to the enzyme and is not removed by anaerobic dialysis or gel permeation
Ni2+
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contains Ni2+-activated alpha subunits, Ni2+ is required for activity and oligomerization
Nickel
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the alphabeta dimer contains approximately 2 mol of nickel per mol of dimer
Nickel
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carbon monoxide dehydrogenase II, enzyme contains 5 metal clusters, a [Ni-4Fe-5S] cluster appears to be the active site of CO oxidation
Nickel
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CO dehydrogenase complex consists of a two-subunit nickel/iron-sulfur component and the two-subunit factor III-containing corrinoid/iron-sulfur (Co/Fe-S) component
Nickel
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the NiFe complex is required for catalyzing the exchange reaction and the acetyl-CoA synthase reaction
Nickel
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both CO and CoASH bind near the nickel site, nickel may therefore be the active metal center for C-C bond formation
Nickel
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the alphabeta dimer contains approximately 2 mol of nickel per mol of dimer
Nickel
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the enzyme contains two metal centers: a Ni-X-[4Fe-4S]2+/1+ cluster, i.e. C-center, that serves as the CO-oxidation site and a standard [4Fe-4S]2+/1+ cluster, i.e. B-center, that mediates electron flow from the C-center to the external electron acceptor, the nickel cation is proposed to be Ni2+ of the oxidized state of the C-center and in the one-electron-reduced state of the C-center, appears to strongly affect the redox behavior of the [4Fe-4S]2+/1+ component of the C-center
Nickel
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wild-type, 0.85 mol per mol of enzyme. Analysis of activation of wild-type and mutant apo-enzymes by nickel
Nickel
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synthesis of an analogue of the C-cluster of Carboxydothermus hydrogenoformans with a planar Ni(II) site and attachment of an exo iron atom in the core unit NiFe4S5 and analysis of products
Zinc
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the alphabeta dimer contains approximately 1 mol of zinc per mol of dimer
Zinc
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the alphabeta dimer contains approximately 1 mol of zinc per mol of dimer
additional information
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no stimulation by MgSO4, CoCl2, NiCl2 and ZnSO4
additional information
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the enzyme contains 30 Fe, 2 Ni, 1 Zn, and 1 Cu (per alpha 2 beta 2 enzyme)
additional information
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no oligomerization or activity in the presence of Co2+, Zn2+, and Cu2+, oligomerization but no exhibition of catalytic activity in the presence of Pd2+ and Pt2+
