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1.2.7.1: pyruvate synthase

This is an abbreviated version!
For detailed information about pyruvate synthase, go to the full flat file.

Word Map on EC 1.2.7.1

Reaction

pyruvate
+
CoA
+
oxidized ferredoxin
=
acetyl-CoA
+
CO2
+
reduced ferredoxin
+ 2 H+

Synonyms

2-ketobutyrate synthase, 2-oxobutyrate (methylviologen), 2-oxobutyrate synthase (benzylviologen), 2-oxobutyrate-ferredoxin oxidoreductase, alpha-ketobutyrate synthase, alpha-ketobutyrate-ferredoxin oxidoreductase, AP120, Ape2126/2128, bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase, EC 1.2.7.2, Moth_0064, NifJ1, OFOR, PFO, PFO A, PFOR, Pfor1, PforA, PFR, PFR1, POR, PorEDABG, pyruvate ferredoxin oxidoreductase, pyruvate-ferredoxin oxidoreductase, pyruvate:Fd oxidoreductase, pyruvate:ferredoxin oxidoreductase, pyruvate:ferredoxin oxidoreductase A, synthase, 2-oxobutyrate, TKV_c04340, Tsac_0046, TTE0445

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.7 With an iron-sulfur protein as acceptor
                1.2.7.1 pyruvate synthase

Storage Stability

Storage Stability on EC 1.2.7.1 - pyruvate synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, anaerobic conditions, no loss of activity of the partially purified enzyme
-20°C, under N2, after addition of 20% (v/v) glycerol to each fraction, the enzyme activity remains nearly constant for several weeks
P80521; P80522; P80523; P80524
-70°C, anaerobically in presence of CoASH, thiamine diphosphate anfd Mg2+, enzyme concentration below 0.02 mg/ml, stable for months
-
2°C, 2 weeks, anaerobic conditions, no loss of activity
4°C, 172 days, 70% residual activity
4°C, storage results in a loss of 80% of the activity within 8 days
P80521; P80522; P80523; P80524