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1.2.4.2: oxoglutarate dehydrogenase (succinyl-transferring)

This is an abbreviated version!
For detailed information about oxoglutarate dehydrogenase (succinyl-transferring), go to the full flat file.

Word Map on EC 1.2.4.2

Reaction

2-oxoglutarate
+
[dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
=
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine
+
CO2

Synonyms

2-ketoglutarate dehydrogenase, 2-OGDH, 2-OGDH E1, 2-OGDH1, 2-OGDH2, 2-oxoadipate dehydrogenase, 2-oxoglutarate decarboxylase, 2-oxoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase complex, 2-oxoglutarate dehydrogenase complex E1 component, 2-oxoglutarate dehydrogenase E1/E2 component, 2-oxoglutarate dehydrogenase-like protein, 2-oxoglutarate:lipoate oxidoreductase, 2OGDH, AKGDH, alpha-ketoglutarate dehydrogenase, alpha-ketoglutarate dehydrogenase complex, alpha-ketoglutarate dehydrogenase multienzyme complex, alpha-ketoglutaric acid dehydrogenase, alpha-ketoglutaric dehydrogenase, alpha-KGD, alpha-KGDH, alpha-KGDHC, alpha-oxoglutarate dehydrogenase, alphaKGDH, At3g55410, dehydrogenase, oxoglutarate, DHTKD1, E1 component of the 2-oxoglutarate dehydrogenase multienzyme complex, E1 subunit of the alpha-ketoglutarate dehydrogenase complex, E1-OGDH1, E1-OGDH2, E1a, E1k, E1o, E1o component, E1o component 2-oxoglutarate dehydrogenase complex, ELK, ilvB, KDH, Kdh E1, ketoglutaric dehydrogenase, KG dehydrogenase, Kgd, KGDH, KGDHC, MPA24.10, OADH, ODH, OdhA, OGDC, OGDH, OGDHC, OGDHL, oxoglutarate decarboxylase, oxoglutarate dehydrogenase, SucA, ThDP-dependent 2-oxoglutarate dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.4 With a disulfide as acceptor
                1.2.4.2 oxoglutarate dehydrogenase (succinyl-transferring)

Purification

Purification on EC 1.2.4.2 - oxoglutarate dehydrogenase (succinyl-transferring)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, HiLoad Q-Sepharose column chromatography, and Superdex S200 gel filtration
by affinity chromatography, purification of OdhI-T14A leads to specific copurification of OdhA, the E1 subunit of ODH
-
enzyme is further purified from commercial pig heart preparation
-
Ni-NTA column chromatography
-
nickel-Sepharose column chromatography
-
partial purification
-
partial purification by 2% and 6% PEG (w/v) precipitations and Sephacryl S-400 gel filtration
-
partial, 2-oxoglutarate dehydrogenase complex
-
partially purified from skeletal muscle of euthermic and hibernating ground squirrels
-
partially, isolation of subsarcolemmal mitochondria from heart
-
poly(ethylene glycol) fractionation and Sephacryl HR300 gel filtration
-
Sephacryl S-500 gel filtration