1.2.3.3: pyruvate oxidase
This is an abbreviated version!
For detailed information about pyruvate oxidase, go to the full flat file.
Word Map on EC 1.2.3.3
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1.2.3.3
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streptococcus
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thiamin
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plantarum
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sanguinis
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pneumococcus
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lipid-activated
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acetohydroxy
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diphosphate-dependent
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phosphotransacetylase
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synthesis
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oralis
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analysis
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medicine
- 1.2.3.3
- streptococcus
- thiamin
- plantarum
- sanguinis
-
pneumococcus
-
lipid-activated
-
acetohydroxy
-
diphosphate-dependent
- phosphotransacetylase
- synthesis
- oralis
- analysis
- medicine
Reaction
Synonyms
acetyl phosphate-producing pyruvate oxidase, phosphate-dependent pyruvate oxidase, POD, POX, POX-2, poxB, PoxF, PyOD, PyrOx, pyruvate oxidase, pyruvate:oxygen-2-oxidoreductase (phosphorylating), pyruvic oxidase, SpxB, SpxB protein
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Engineering
Engineering on EC 1.2.3.3 - pyruvate oxidase
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P178S/S188N/A458V
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stabilizes quaternary rather than tertiary structure
V265A
additional information
V265A
similar catalytic properties compared to the wild-type protein, but decreased affinity for FAD
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poxB mutant, absence of pyruvate oxidase activity, which reduces glucose uptake rate, biomass yield, acetate production and cell growth rate, and increases O2 consumption and CO2 evolution. Glucokinase activity increases, whereas the flux through glycolysis is reduced due to down-regulation of other glycolytic enzymes such as 6-phosphofructosekinase and fructose bisphosphate aldolase. TCA cycle enzymes such as citrate synthase and malate dehydrogenase are repressed in the poxB mutant when cells are cultivated in LB medium. Activation of glucose-6-phosphate dehydrogenase and acetyl-CoA synthetase
additional information
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enzyme deletion mutant, 20-25% decrease in acetate production during gr0wth on glucose as sole carbon source, 60-80% decrease in acetate production during growth on maltose, constitutive overproduction of enzyme, acetate becomes the only fermentation end product
additional information
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no pyruvate oxidase activity detectable in a PoxB/PoxF double mutant
additional information
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enzyme deletion mutant, 20-25% decrease in acetate production during gr0wth on glucose as sole carbon source, 60-80% decrease in acetate production during growth on maltose, constitutive overproduction of enzyme, acetate becomes the only fermentation end product
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additional information
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a cidC pyruvate oxidase mutant exhibited decreased pyruvate oxidase activity and shows reduced cell lysis in stationary phase
additional information
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a cidC pyruvate oxidase mutant exhibited decreased pyruvate oxidase activity and shows reduced cell lysis in stationary phase
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additional information
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SpxB-deficient mutant, shows drastically diminished H2O2 production
additional information
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SpxB-deficient mutant, shows drastically diminished H2O2 production
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