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1.2.3.14: abscisic-aldehyde oxidase

This is an abbreviated version!
For detailed information about abscisic-aldehyde oxidase, go to the full flat file.

Word Map on EC 1.2.3.14

Reaction

abscisic aldehyde
+
H2O
+
O2
=
abscisate
+
H2O2

Synonyms

AAO, AAO1, AAO2, AAO3, ABA aldehyde oxidase, ABA-specific aldehyde oxidase, abscisic aldehyde oxidase, abscisic aldehyde oxidase 3, AO-3, AO3, AOd, AOdelta, Arabidopsis aldehyde oxidase 3, EC 1.2.3.1

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.3 With oxygen as acceptor
                1.2.3.14 abscisic-aldehyde oxidase

Substrates Products

Substrates Products on EC 1.2.3.14 - abscisic-aldehyde oxidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthaldehyde + H2O + O2
naphthalene-1-carboxylate + H2O2
show the reaction diagram
-
substrate activity assay
-
-
?
abscisic aldehyde + 2,6-dichloroindophenol
abscisic acid + H2O2
show the reaction diagram
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
-
?
abscisic aldehyde + H2O + O2
abscisate + H2O2
show the reaction diagram
benzaldehyde + 2,6-dichloroindophenol
benzoic acid + H2O2
show the reaction diagram
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
-
?
heptaldehyde + 2,6-dichloroindophenol
heptanoic acid + H2O2
show the reaction diagram
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
-
?
indole-3-aldehyde + H2O + O2
indole-3-carboxylate + H2O2
show the reaction diagram
indole-3-carbaldehyde + 2,6-dichloroindophenol
indole-3-carboxylate + H2O2
show the reaction diagram
2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen
rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2-
-
?
NADH + O2
NAD+ + O2-
show the reaction diagram
oxidation of NADH by AAO1 and AAO3, no oxidation of NADPH by AAO1 or AAO3
for confirmation, O2--dependent reduction of cytochrome c monitored, oxidation of NADH by AAO1 and AAO3 does not result in detectable levels of H2O2
-
?
additional information
?
-
confirmation of superoxide generation by AAO1 and AAO3 by monitoring the reduction of the tetrazolium salt XTT due to O2-
-
-
?