1.2.1.85: 2-hydroxymuconate-6-semialdehyde dehydrogenase

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For detailed information about 2-hydroxymuconate-6-semialdehyde dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-hydroxymuconate semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde dehydrogenase, 5C-2HMS dehydrogenase, HMS dehydrogenase, NAD+-dependent 2-hydroxy-muconic 6-semialdehyde dehydrogenase, NahI, praB, XylG

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.85 2-hydroxymuconate-6-semialdehyde dehydrogenase

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Results	in table
780	AA Sequence
3	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
7	General Information
6	Inhibitors
10	kcat/KM [mM/s]
12	KM Value [mM]
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
55	Organism
12	Pathway
3	pH Optimum
3	Purification (Commentary)
1	Reaction
12	Reference
4	Source Tissue
1	Specific Activity [micromol/min/mg]
25	Substrates and Products (Substrate)
6	Subunits
29	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.85 - 2-hydroxymuconate-6-semialdehyde dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography

Pseudomonas putida

-

390225

recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, and another nickel affinity chromatography step, followed by gel filtration and ultrafiltration

Pseudomonas putida

Q1XGK8

741776

streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography

Bordetella sp.

-

672682