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0.00000054 - 3.7
3-aminopropionaldehyde
0.0027
3-dimethylsulfoniopropionaldehyde
-
-
0.035 - 0.34
3-N-trimethylaminopropionaldehyde
0.00196 - 1.1
4-Aminobutyraldehyde
0.000024 - 0.00005
4-guanidinobutyraldehyde
0.0078 - 0.041
4-N-trimethylaminobutyraldehyde
0.0014
4-trimethylaminobutyraldehyde
-
cytoplasmic enzyme
0.000005 - 14.8
Betaine aldehyde
11 - 48
D,L-glyceraldehyde
3.8
formaldehyde
-
pH 7.7, coenzyme NAD+
0.13
glycine betaine aldehyde
-
-
0.152 - 11.5
glycolaldehyde
0.2 - 15
Isovaleraldehyde
0.5
p-nitrobenzaldehyde
-
pH 7.7, coenzyme NAD+
51 - 140
phenyl acetaldehyde
additional information
additional information
-
0.00000054
3-aminopropionaldehyde
-
pH 8.7, 37°C
0.0012
3-aminopropionaldehyde
-
isozyme BADH2, pH and temperature not specified in the publication
0.004
3-aminopropionaldehyde
-
0.017
3-aminopropionaldehyde
-
isozyme BADH1, pH and temperature not specified in the publication
0.453
3-aminopropionaldehyde
-
at pH 8.0 and 30°C
3.7
3-aminopropionaldehyde
in 100 mM sodium diphosphate pH 8.0, at 22°C
0.035
3-N-trimethylaminopropionaldehyde
-
isozyme BADH1, pH and temperature not specified in the publication
0.0914
3-N-trimethylaminopropionaldehyde
-
at pH 8.0 and 30°C
0.34
3-N-trimethylaminopropionaldehyde
-
isozyme BADH2, pH and temperature not specified in the publication
0.00196
4-Aminobutyraldehyde
-
at pH 8.0 and 30°C
0.003
4-Aminobutyraldehyde
-
wild-type enzyme
0.004
4-Aminobutyraldehyde
-
mitochondrial enzyme
0.005
4-Aminobutyraldehyde
-
-
0.0098
4-Aminobutyraldehyde
-
mutant enzyme E103Q
0.024
4-Aminobutyraldehyde
-
cytoplasmic enzyme
0.037
4-Aminobutyraldehyde
-
-
0.049
4-Aminobutyraldehyde
-
1.1
4-Aminobutyraldehyde
in 100 mM sodium diphosphate pH 8.0, at 22°C
0.000024
4-guanidinobutyraldehyde
-
pH 8.7, 37°C
0.00005
4-guanidinobutyraldehyde
-
pH 8.7, 37°C
0.0078
4-N-trimethylaminobutyraldehyde
-
isozyme BADH1, pH and temperature not specified in the publication
0.0221
4-N-trimethylaminobutyraldehyde
-
at pH 8.0 and 30°C
0.041
4-N-trimethylaminobutyraldehyde
-
isozyme BADH2, pH and temperature not specified in the publication
0.014
acetaldehyde
-
-
0.014
acetaldehyde
-
mitochondrial enzyme
0.115
acetaldehyde
-
cytoplasmic enzyme
0.13
acetaldehyde
-
isozyme BADH1, pH and temperature not specified in the publication
0.17
acetaldehyde
-
isozyme BADH2, pH and temperature not specified in the publication
3.6
acetaldehyde
-
pH 7.7, coenzyme NAD+
28
acetaldehyde
-
pH 7.7, coenzyme NADP+
0.1
benzaldehyde
-
pH 7.7, coenzyme NAD+
6.7
benzaldehyde
-
pH 7.7, coenzyme NADP+
0.000005
Betaine aldehyde
-
pH 8.7, 37°C
0.0561
Betaine aldehyde
-
-
0.065
Betaine aldehyde
-
mutant enzyme E103Q
0.068
Betaine aldehyde
-
wild-type enzyme
0.07716
Betaine aldehyde
-
-
0.09
Betaine aldehyde
mutant enzyme A441C, at pH 8.0 and 30°C
0.0937
Betaine aldehyde
-
-
0.098
Betaine aldehyde
wild type enzyme, at pH 8.0 and 30°C
0.109
Betaine aldehyde
-
at pH 8.0 and 30°C
0.11
Betaine aldehyde
-
-
0.11
Betaine aldehyde
-
mutant enzyme G234T, at pH 8.0 and 30°C
0.118
Betaine aldehyde
-
mitochondrial enzyme
0.119
Betaine aldehyde
mutant enzyme A441S, at pH 8.0 and 30°C
0.12
Betaine aldehyde
-
mutant enzyme G234A, at pH 8.0 and 30°C
0.123
Betaine aldehyde
-
cytoplasmic enzyme
0.127
Betaine aldehyde
-
-
0.133
Betaine aldehyde
-
-
0.1455
Betaine aldehyde
-
-
0.15
Betaine aldehyde
-
pH 7.5, 20°C, native betaine-aldehyde dehydrogenase or betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein
0.16
Betaine aldehyde
-
-
0.17
Betaine aldehyde
at pH 8.0 and 30°C
0.17
Betaine aldehyde
-
wild type enzyme, at pH 8.0 and 30°C
0.17
Betaine aldehyde
-
mutant enzyme G234S, at pH 8.0 and 30°C
0.18
Betaine aldehyde
mutant enzyme A441T, at pH 8.0 and 30°C
0.208
Betaine aldehyde
-
-
0.23
Betaine aldehyde
-
isozyme BADH2, pH and temperature not specified in the publication
0.26
Betaine aldehyde
-
-
0.26
Betaine aldehyde
-
mutant enzyme H448F, at pH 8.0 and 30°C
0.31
Betaine aldehyde
-
-
0.38
Betaine aldehyde
-
-
0.434
Betaine aldehyde
-
30°C, pH 8.0, cosubstrate NADP+
0.506
Betaine aldehyde
-
30°C, pH 8.0, cosubstrate NAD+
0.512
Betaine aldehyde
mutant enzyme A441V, at pH 8.0 and 30°C
0.57
Betaine aldehyde
-
mutant enzyme H448F/P449M, at pH 8.0 and 30°C
0.605
Betaine aldehyde
mutant enzyme A441F, at pH 8.0 and 30°C
0.665
Betaine aldehyde
pH 8.0, 30°C, recombinant isozyme LrAMADH1
0.91
Betaine aldehyde
-
mutant enzyme Q162M, at pH 8.0 and 30°C
1.18
Betaine aldehyde
-
mutant enzyme H448F/Y450L, at pH 8.0 and 30°C
1.791
Betaine aldehyde
mutant enzyme A441I, at pH 8.0 and 30°C
1.8
Betaine aldehyde
-
pH 7.7, coenzyme NADP+
1.92
Betaine aldehyde
-
mutant enzyme Y450L, at pH 8.0 and 30°C
1.94
Betaine aldehyde
-
mutant enzyme P449M/Y450L, at pH 8.0 and 30°C
2.16
Betaine aldehyde
-
mutant enzyme L161M7Q162M, at pH 8.0 and 30°C
2.6
Betaine aldehyde
-
isozyme BADH1, pH and temperature not specified in the publication
4.61
Betaine aldehyde
-
mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C
4.76
Betaine aldehyde
-
mutant enzyme V288D, at pH 8.0 and 30°C
6.5
Betaine aldehyde
-
pH 7.7, coenzyme NAD+
6.81
Betaine aldehyde
in 100 mM sodium diphosphate pH 8.0, at 22°C
13.8
Betaine aldehyde
-
mutant enzyme S290T, at pH 8.0 and 30°C
14.8
Betaine aldehyde
-
mutant enzyme W456H, at pH 8.0 and 30°C
0.5
Butyraldehyde
-
pH 7.7, coenzyme NAD+
3.4
Butyraldehyde
-
pH 7.7, coenzyme NADP+
11
D,L-glyceraldehyde
-
pH 7.7, coenzyme NADP+
48
D,L-glyceraldehyde
-
pH 7.7, coenzyme NAD+
0.152
glycolaldehyde
-
mitochondrial enzyme
4.3
glycolaldehyde
-
pH 7.7, coenzyme NAD+
11.5
glycolaldehyde
-
pH 7.7, coenzyme NADP+
0.2
Isovaleraldehyde
-
pH 7.7, coenzyme NAD+
15
Isovaleraldehyde
-
pH 7.7, coenzyme NADP+
2.5
methylglyoxal
-
pH 7.7, coenzyme NAD+
9
methylglyoxal
-
pH 7.7, coenzyme NADP+
0.0025
NAD+
-
pH 7.7, reaction with benzaldehyde
0.0028
NAD+
mutant enzyme A441I, at pH 8.0 and 30°C
0.004
NAD+
-
pH 7.7, reaction with isovaleraldehyde
0.0064
NAD+
mutant enzyme A441V, at pH 8.0 and 30°C
0.00766
NAD+
-
at pH 8.0 and 30°C
0.009
NAD+
-
pH 7.7, reaction with undecanal
0.011
NAD+
-
reaction with betaine aldehyde
0.012
NAD+
-
pH 7.7, reaction with valeraldehyde
0.0133
NAD+
-
reaction with 3-dimethylsulfoniopropionaldehyde
0.014
NAD+
mutant enzyme A441C, at pH 8.0 and 30°C
0.018
NAD+
mutant enzyme A441F, at pH 8.0 and 30°C
0.019
NAD+
-
wild-type enzyme, reaction with betaine aldehyde
0.022
NAD+
wild type enzyme, at pH 8.0 and 30°C
0.023
NAD+
-
mutant enzyme E103Q, reaction with betaine aldehyde
0.024
NAD+
-
mutant enzyme E103Q, reaction with 4-aminobutyraldehyde
0.024
NAD+
mutant enzyme A441T, at pH 8.0 and 30°C
0.026
NAD+
-
wild-type enzyme, reaction with 4-aminopropionaldehyde
0.028
NAD+
-
cytoplasmic enzyme
0.029
NAD+
mutant enzyme A441S, at pH 8.0 and 30°C
0.034
NAD+
-
mitochondrial enzyme
0.05
NAD+
-
pH 7.7, reaction with phenyl acetaldehyde
0.083
NAD+
-
30°C, pH 8.0
0.12
NAD+
-
mutant enzyme G234S, at pH 8.0 and 30°C
0.136
NAD+
-
reaction with 4-aminobutyraldehyde
0.26
NAD+
-
wild type enzyme, at pH 8.0 and 30°C
0.27
NAD+
-
mutant enzyme V288D, at pH 8.0 and 30°C
0.3
NAD+
-
mutant enzyme G234T, at pH 8.0 and 30°C
0.34
NAD+
-
mutant enzyme H448F/Y450L, at pH 8.0 and 30°C
0.38
NAD+
-
mutant enzyme W456H, at pH 8.0 and 30°C
0.41
NAD+
-
mutant enzyme S290T, at pH 8.0 and 30°C
0.42
NAD+
-
mutant enzyme H448F, at pH 8.0 and 30°C
0.43
NAD+
at pH 8.0 and 30°C
0.43
NAD+
-
mutant enzyme P449M/Y450L, at pH 8.0 and 30°C
0.68
NAD+
-
mutant enzyme Q162M, at pH 8.0 and 30°C
0.71
NAD+
-
mutant enzyme L161M7Q162M, at pH 8.0 and 30°C
0.8
NAD+
-
pH 7.7, reaction with p-nitrobenzaldehyde
1.01
NAD+
-
mutant enzyme Y450L, at pH 8.0 and 30°C
1.12
NAD+
-
mutant enzyme G234A, at pH 8.0 and 30°C
1.12
NAD+
-
mutant enzyme H448F/P449M, at pH 8.0 and 30°C
3.42
NAD+
-
mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C
0.08
NADP+
-
pH 7.7, reaction with undecanal
0.27
NADP+
-
pH 7.7, reaction with valeraldehyde
0.31
NADP+
-
pH 7.7, reaction with isovaleraldehyde
0.385
NADP+
-
30°C, pH 8.0
0.61
NADP+
-
pH 7.7, reaction with phenyl acetaldehyde
1
NADP+
-
pH 7.7, reaction with p-nitrobenzaldehyde
1.4
NADP+
-
pH 7.7, reaction with benzaldehyde
2.26
NADP+
at pH 8.0 and 30°C
3.68
NADP+
-
at pH 8.0 and 30°C
51
phenyl acetaldehyde
-
pH 7.7, coenzyme NADP+
140
phenyl acetaldehyde
-
pH 7.7, coenzyme NAD+
0.8
propionaldehyde
-
pH 7.7, coenzyme NAD+
21
propionaldehyde
-
pH 7.7, coenzyme NADP+
2 - 3
Undecanal
-
pH 7.7, coenzyme NADP+
106
Undecanal
-
pH 7.7, coenzyme NAD+
0.2
Valeraldehyde
-
pH 7.7, coenzyme NAD+
4.1
Valeraldehyde
-
pH 7.7, coenzyme NADP+
additional information
additional information
-
for the mutants and wild-type enzymes KM NADP+ varies within 0.060 and 0.107 mM, KM NAD+ within 0.254 and 0.411 mM, KM betaine aldehyde within 0.270 and 0.434 mM
-
additional information
additional information
Michaelis-Menten kinetics, except for LrAMADH1 on 4-aminobutyraldehyde
-
additional information
additional information
-
pkBADH kinetic analysis
-
additional information
additional information
thermodynamcis
-
additional information
additional information
-
thermodynamcis
-
1.2.1.8 betaine-aldehyde dehydrogenase
results (
results (
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