Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.2.1.8: betaine-aldehyde dehydrogenase

This is an abbreviated version!
For detailed information about betaine-aldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.8

Reaction

Betaine aldehyde
+
NAD+
 +
H2O
=
betaine
 +
NADH
 + 2 H+

Synonyms

AcBADH, ALD10, aldehyde dehydrogenase, ALDH10, ALDH10A8, ALDH10A9, AMADH, AMADH1, aminoaldehyde dehydrogenase, BADH, BADH1, BADH2, badh2-E2, badh2-E7, betaine aldehyde dehydrogenase, betaine aldehyde dehydrogenase 1, betaine aldehyde dehydrogenase 1, chloroplastic, betaine aldehyde dehydrogenase 2, betaine aldehyde dehydrogenase, chloroplastic, betaine aldehyde oxidase, betaine aldehyde: NAD(P)+ oxidoreductase, betaine aldehyde: NAD+ oxidoreductase, betaine aldehyde:NAD(P)+ oxidoreductase, betaine aldehyde:NAD+ oxidoreductase, betaine-aldehyde dehydrogenase, BetB, dehydrogenase, betaine aldehyde, JcBD1, LcBADH, More, OsALDH10-1, OsALDH10-2, OsBADH1, OsBADH2, PaBADH, PaBADH2, pkBADH, PND BADH2, SACOL2628, YdcW, ZBD1, Zoysia betaine aldehyde dehydrogenase 1

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.8 betaine-aldehyde dehydrogenase

Cloned

Cloned on EC 1.2.1.8 - betaine-aldehyde dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in different strains (A764, A765, A767 and YPIC3) of Pichia pastoris
construction of a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein and expression in Escherichia coli and Nicotiana tabacum. Escherichia coli cells expressing the fusion protein are able to grow to higher final densities and to accumulate more glycine betaine than cells expressing choline dehydrogenase alone
-
expressed in Arabidopsis thaliana
expressed in Escherichia coli
expressed in Escherichia coli BL21
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Gold cells
-
expressed in Escherichia coli BL21(DE3)/pMAGIC cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli strain BL21
-
expressed in Ipomoea batatas cultivar Sushu-2 via Agrobacterium-mediated transformation
-
expressed in Nicotiana tabacum
expressed in trifoliate orange Poncirus trifoliata by Agrobacterium-mediated transformation
-
expression in Escherichia coli
-
expression in Escherichia coli and Salmonella typhimurium
-
expression in Escherichia coli cells
-
expression in Nicotiana tabacum 89. Tobacco expressing BADH survives on Murashige-Skoog medium containing 200 mM NaCl, whereas the untransformed plants turn yellow after 20 d and die
For OsBADH2, preliminary experiments based on RT-PCR show that the mRNA is expressed constitutively and multiple transcription products ae detected. Primers specific to the 5' region are used. To analyze the transcripts derived from the OsBADH2 gene, seedlings from different varieties under different rowth conditions are harvested for the total RNA isolation. As a result, all the 59 cDNA clones sequenced also have deletions at the 5' exonic region. Similar to that in the OsBADH1 gene, various unusual events in the OsBADH2 locus generate a number of truncated transcripts. The size of the deleted sequences from 5' UTR and exon(s) range from 112 to 523 nucleotides.
gene AMADH1, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time PCR enzyme expression analysis
gene BADH, Agrobacterium tumefaciens-mediated recombinant expression in Arabidopsis thaliana
gene BADH, Agrobacterium tumefaciens-mediated recombinant expression in Cichorium intybus and in Triticum aestivum
gene BADH, Agrobacterium tumefaciens-mediated recombinant expression in Solanum tuberosum and in Populus nigra
gene BADH, Agrobacterium tumefaciens-mediated recombinant expression in Trachyspermum ammi, Nicotiana tabacum, and Solanum lycopersicum, as well as in Juglans regia
gene BADH, BADH2 encodes on chromosome 8, sequence comparisons
gene BADH, microprojectile bombardment method-based transfection of Triticum aestivum
gene BADH, phylogenetic analysis, recombinant expression in Zea mays, quantitative RT-PCR expression analysis
gene BADH, recombinant expression in Zea mays via pollen-tube pathway
gene BADH, recombinant expression of AcBADH in transgenic Glycine max cv. Jinong 17 plants using the transfection method via Agrobacterium tumefaciens strain LBA4404, quntitative RT-PCR expression analysis
gene BADH, sequence comparisons
gene BADH, sequence comparisons, heterologous expression of a BADH gene from Ammopiptanthus nanus in Escherichia coli validates its role in abiotic tolerance
-
gene BADH, sequence comparisons, recombinant expression of the enzyme in Escherichia coli strain ER2566
gene BADH1, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain M15 from vector pREP4 under the control of an inducible promoter
Halalkalibacterium halodurans
gene BADH1, sequence comparisons
gene BADH2, reconstructed from transcriptome, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis
high-level expression of betaine aldehyde dehydrogenase in cultured cells, roots, and leaves of carrot via plastid genetic engineering. Homoplasmic transgenic plants that exhibit high levels of salt tolerance are regenerated from bombarded cell cultures via somatic embryogenesis. Transformation efficiency of carrot somatic embryos is very high, with one transgenic event per approximately seven bombarded plates under optimal conditions. In vitro transgenic carrot cells transformed with the badh transgene are visually green in color when compared to untransformed carrot cells, this offers a visual selection for transgenic lines. Transgenic carrot plants expressing BADH grow in presence of high concentrations of NaCl, up to 400 mM
-
in Escherichia coli strain BL21 (DE3)
in Nicotiana tabacum, the BADH gene isolating from spinach is used to construct a vector plasmid that contained the promoter for 35S ribosomal RNA from CaMV35S, the sequence encoding the transit peptide of the small subunit of Rubisco of tobacco and the terminator of the gene for nopaline synthase. Nicotiana tabacum (wild type K326) is transformed with the resultant plasmid by the standard Agrobacterium-mediated method and five independent lines of transgenic tobacco plants are established. Transformed plants are used as the source of plant materials. The role of glycine betaine in vivo in protecting photosynthesis from salt stress is investigated.
-
into the pCR2.1-TOPO and pGEX-6p-2 vector for sequencing of the coding region and expression of the enzyme in Escherichia coli BL21, respectively
into the pGEM-T easy vector for sequencing
mutant enzyme E103Q expressed in Escherichia coli
-
of genes encoding five forms of spinach enzyme: full length, mature, mutant E103Q, mutant E103K and chimera
-
overexpression in Escherichia coli
-
partial characterization of the full-length BADH promoter of Suaeda liaotungensis through fusions of various lengths fused to the beta-glucuronidase coding sequence and subsequent expression in tobacco leaves treatment with NaCl are reported
-
plasmid pCALbetB containing the full sequence of the gene betB that encodes PaBADH is used for the expression of the enzyme in Escherichia coli cells
Seeds from T2 generation (lines T2-1, T2-3, T2-5, T2-8 and T2-9) of transgenic tomato containing the BADH gene from Atriplex hortensis were used
-
the BADH gene of Leymus chinensis is cloned by RT-PCR and RACE technology and is designated as LcBADH. The cDNA sequence of LcBADH is 1774 bp including the open reading frame of 1521 bp (coding 506 amino acids). The vector of prokaryotic expression is constructed by inserting the LcBADH gene fragment into pET30a(+) and transformed into Escherichia coli BL21(DE3)
-
the gene betB, encoding for Pseudomonas aeruginosa betaine-aldehyde dehydrogenase, is cloned into a pCAL-n vector for sequencing and protein expression in Escherichia coli
To compare the posttranscriptional processing patterns of the BADH homologs between cereal crop species and more distantly related dicotyledonous species, RT-PCR experiments using total RNA extracted from seedlings of spinach are conducted. Primers designed to amplify the full length of mRNA of BADH homologs are used. As anticipated, the RT-PCR products of BADH homologs from Arabidopsis are of expected size for correctly processed transcripts. Sequencing analysis of 4 cDNA clones confirms the correct processing.
To compare the posttranscriptional processing patterns of the BADH homologs between cereal crop species and more distantly related dicotyledonous species, RT-PCR experiments using total RNA extracted from seedlings of spinach are conducted. Primers designed to amplify the full length of mRNA of BADH homologs are used. As anticipated, the RT-PCR products of BADH homologs from spinach are of expected size for correctly processed transcripts. Sequencing analysis of 4 cDNA clones confirms the correct processing.
To compare the posttranscriptional processing patterns of the BADH homologs between cereal crop species and more distantly related dicotyledonous species, RT-PCR experiments using total RNA extracted from seedlings of spinach are conducted. Primers designed to amplify the full length of mRNA of BADH homologs are used. As anticipated, the RT-PCR products of BADH homologs from tomato are of expected size for correctly processed transcripts. Sequencing analysis of 3 cDNA clones confirms the correct processing.
-
To determine whether the unusual events occurring in the BADH transcripts are specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings of maize species are carried out. These experiments used primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. The sequencing data from a total of 6 cDNA clones demonstrate that all the tested cDNA clones have deletion(s) of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
Q53CF4
To determine whether the unusual events occurring in the transcripts are specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings of barley are performed. These experiments use primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. The sequencing data from a total of 5 cDNA clones demonstrate that all the tested cDNA clones have deletions of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
To determine whether the unusual events occurring in the transcripts are specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings of maize are performed. These experiments use primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. The sequencing data from a total of 9 cDNA clones demonstrate that all the tested cDNA clones have deletions of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
Q53CF4
To determine whether the unusual events occurring in the transcripts are specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings of wheat are performed. These experiments use primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. Sequencing data from 22 DNA clones demonstrate that all the tested cDNA clones had deletion(s) of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
To determine whether the unusual events occurring in the transcripts are specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings wheat are performed. These experiments use primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. Sequencing data from 4 DNA clones demonstrate that all the tested cDNA clones had deletion(s) of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
To determine whether the unusual events occurring in the transcripts were specific only to the rice genome, RT-PCR experiments using the total RNA extracted from seedlings of barley are performed. These experiments use primers either to amplify the full length of mRNA or exclusively the 5' region of BADH homologs corresponding to those in rice. The sequencing data from a total of 6 cDNA clones demonstrate that all the tested cDNA clones have deletions of the 5' exonic sequences resulting from the unusual posttranscriptional processing.
to examine whether the expressed products are OsBADh1 gene, the RT-PCR-amplified fragments are cloned and sequenced. Primers derived from 5' and 3' untranslated regions are used to isolate the full length of OsBADH1 cDNA clones. Resultant sequencing analysis reveal that the cDNAs are truncated at the 5' exonic region. Observed expression products are shorter than the expected size of 695 bp of the 5' exonic region. Sequence comparison of the cDNAs reveal a considerable variation in their structural compositions. All of the cDNAs contain a deletion of the 5' coding sequence within the OsBADH1 gene. The deleted exon material ranged from 28 to 225 nucleotides in size. The start-point of the deletions in four cDNAs begin with the first nucleotide of the coding sequence, which give rise to the loss of translation initiation codon. 32 cDNAs encode derivatives with frame-shifts in the open reading frame , introducing various stop codons at different positions. Only 5 cDNA clones show the potential to encode partial BADH1 proteins with deletions that code for a part of the putative NAD+ binding domain. Most of the missing sequences from the truncated transcripts indicate above involved 2 different exons, and in a few cases the truncation take place within a single exon. In addition, two independent deletions of exon materials within a single cDNA clone are observed in 5 clones. Therefore, no cDNA is found to have the capacity to encode the full length of the OsBADH1 protein, indicating that correctly processed transcripts represent a very small proportion of the total cytoplasmic mature OsBADH1 RNA population and consequently that the majority of the OsBADH1 mRNAs are unlikely to encode functional proteins.
wild type Pseudomonas aeruginosa betaine aldehyde dehydrogenase and the four mutants C353A, C377A, C439A and C286A
-