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1.2.1.28: benzaldehyde dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about benzaldehyde dehydrogenase (NAD+), go to the full flat file.

Reaction

benzaldehyde
+
NAD+
+
H2O
=
benzoate
+
NADH
+
H+

Synonyms

BALDH, benzaldehyde dehydrogenase, benzaldehyde dehydrogenase (NAD), BZDH, mdlD, More, NAD(P)-dependent benzaldehyde dehydrogenase, PpBADH, XylC

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.28 benzaldehyde dehydrogenase (NAD+)

Purification

Purification on EC 1.2.1.28 - benzaldehyde dehydrogenase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration
-
by affinity chromatography on Ni-NTA agarose
-
dehydrogenases I and II
-
recombinant enzyme 14fold from Escherichia coli strain BL21(DE3) by anion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
soluble proteins are extracted from elicitor-treated cell cultures
-
to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography
-