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1.2.1.22: lactaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about lactaldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.22

Reaction

(S)-lactaldehyde
+
NAD+
+
H2O
=
(S)-lactate
+
NADH
+
H+

Synonyms

ALD, AldA, aldehyde dehyrogenase, ALDH, AN0544, AvLADH, dehydrogenase, lactaldehyde, L-lactaldehyde dehydrogenase, L-lactaldehyde:NAD oxidoreductase, Lactaldehyde dehydrogenase, LADH, MJ1411, More, NAD-dependent lactaldehyde dehydrogenase, nicotinamide adenine dinucleotide (NAD)-linked dehydrogenase, PsLADH, SSO3117

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.22 lactaldehyde dehydrogenase

Purification

Purification on EC 1.2.1.22 - lactaldehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion-exchange chromatography
by gel filtration
mutant F180T purified to homogeneity (more than 95% pure), on Ni2+ resin
-
Ni-affinity chromatography
-
nickel-chelating affinity column
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography to near homogeneity
three chromatography steps