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1.2.1.16: succinate-semialdehyde dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about succinate-semialdehyde dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.2.1.16

Reaction

succinate semialdehyde
+
NAD(P)+
+
H2O
=
succinate
+
NAD(P)H
+
H+

Synonyms

3-sulfopropanal dehydrogenase, ALDH, BADH, BsSSADH, cce4228, cce4228 protein, dehydrogenase, succinate semialdehyde (nicotinamide adenine dinucleotide (phosphate)), gabD, GabD dehydrogenase, GabD1, gapN-2, More, NAD(P)+-coupled 3-sulfopropanal dehydrogenase, NAD(P)+-dependent benzaldehyde dehydrogenase, NAD(P)+-dependent succinate semialdehyde dehydrogenase, NAD(P)+-dependent succinic semialdehyde dehydrogenase, NAD(P)-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent SSADH, SpSSADH, SSA dehydrogenase, SSADH, SSADH-II, SSADHase, SSDH, SSO1842, ST0064, succinate semialdehyde dehydrogenase, succinate semialdehyde dehydrogenase (nicotinamide adenine dinucleotide (phosphate)), succinate-semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase (NAD(P)), succinate-semialdehyde dehydrogenase (NAD(P)+), succinic semialdehyde dehydrogenase, succinic semialdehyde:NAD(P)+ oxidoreductase, sulfopropanal dehydrogenase, YneI

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.16 succinate-semialdehyde dehydrogenase [NAD(P)+]

Crystallization

Crystallization on EC 1.2.1.16 - succinate-semialdehyde dehydrogenase [NAD(P)+]

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo-form and in complex with NAD+, hanging drop vapor diffusion method, using 2 M sodium formate and 0.1 M sodium acetate (pH 4.6), at 22°C
structures of apoenzyme and in complex with NAD+, to 1.85 and 1.9 A resolution. Two domain protein with the active site located in the interdomain interface. The NAD+ molecule is bound in the long channel with its nicotinamide ring positioned close to the side chain of the catalytic Cys268
apoenzyme and in complex with NAD+, hanging drop vapor diffusion method, using 0.1 M MES monohydrate pH 5.8-6.1 and 18-23% (w/v) polyethylene glycol 4000
-
purified recombinant SpSSADH in apo-form and in a binary complex with NADP+, hanging drop vapor diffusion method, for the apoform crystals: mixing 0.001 ml of protein solution with 0.001 ml of reservoir solution containing 0.1 M MES monohydrate, pH 5.8-6.1, and 18-23% w/v PEG 4000, and equilibration over 0.5 ml reservoir solution, for the binary complex crystals: mixing of protein solution with NADP+ in a 1:10 molar ratio with reservoir solution containing 0.1 M sodium acetate trihydrate, pH 4.6, and 2 M ammonium sulfate, 22°C, X-ray diffraction structure determination and analysis at 1.6 A and 2.1 A resolutions, respectively, molecular replacement method using the structure of Escherichia coli SSADH, PDB ID 3JZ4, as search model