1.2.1.16: succinate-semialdehyde dehydrogenase [NAD(P)+] This is an abbreviated version! For detailed information about succinate-semialdehyde dehydrogenase [NAD(P)+], go to the full flat file .
Reaction
succinate semialdehyde +
NAD(P)+ +
H2O =
succinate +
NAD(P)H +
H+
Synonyms 3-sulfopropanal dehydrogenase, ALDH, BADH, BsSSADH, cce4228, cce4228 protein, dehydrogenase, succinate semialdehyde (nicotinamide adenine dinucleotide (phosphate)), gabD, GabD dehydrogenase, GabD1, gapN-2, More, NAD(P)+-coupled 3-sulfopropanal dehydrogenase, NAD(P)+-dependent benzaldehyde dehydrogenase, NAD(P)+-dependent succinate semialdehyde dehydrogenase, NAD(P)+-dependent succinic semialdehyde dehydrogenase, NAD(P)-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent SSADH, SpSSADH, SSA dehydrogenase, SSADH, SSADH-II, SSADHase, SSDH, SSO1842, ST0064, succinate semialdehyde dehydrogenase, succinate semialdehyde dehydrogenase (nicotinamide adenine dinucleotide (phosphate)), succinate-semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase (NAD(P)), succinate-semialdehyde dehydrogenase (NAD(P)+), succinic semialdehyde dehydrogenase, succinic semialdehyde:NAD(P)+ oxidoreductase, sulfopropanal dehydrogenase, YneI
ECTree
Cofactor
Cofactor on EC 1.2.1.16 - succinate-semialdehyde dehydrogenase [NAD(P)+]
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NAD+
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-
NAD+
-
preferred cofactor
NAD+
-
preferred cofactor
NAD+
-
rate with NADP+ is about twice that with NAD+, crude extract
NAD+
-
enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+
NAD+
-
NADP+ is about 8.2 times as active as NAD+
NAD+
-
2.5fold better activity than with NADP+ at 0.2 mM concentration
NAD+
-
higher activity in the presence of NAD+ than in the presence of NADP+
NAD+
kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+
NAD+
-
long incubations lead to a modest utilization of NAD+ as substrate
NAD+
the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher
NAD+
affinity to NAD+ is 10 times higher than to NADP+
NAD+
similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors
NADP+
-
-
NADP+
-
20% of the activity with NAD+
NADP+
-
preferred cofactor
NADP+
-
rate with NADP+ is about twice that with NAD+, crude extract
NADP+
-
reduces NAD+ at one-twelfth the rate of NADP+
NADP+
-
NADP+ is 8.2 times as active as NAD+
NADP+
-
enzyme is active both with NADP+ and NAD+, Vmax with NAD+ is considerably larger than that obtained with NADP+
NADP+
-
NAD+ is preferred over NADP+
NADP+
-
less active with NADP+ compared to NAD+
NADP+
-
GabD dehydrogenase is specific for NADP+
NADP+
-
higher activity in the presence of NAD+ than in the presence of NADP+
NADP+
-
at 9% of the rate with NAD+
NADP+
-
although long incubations lead to a modest utilization of NAD+ as substrate, NADP+ is the preferred substrate
NADP+
kcat/Km-value is comparable for succinate semialdehyde with NADP+ and for succinate semialdehyde with NAD+
NADP+
the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher
NADP+
affinity to NAD+ is 10 times higher than to NADP+
NADP+
similar values of the catalytic efficiency (kcat/Km) with both NAD+ and NADP+ as cofactors
NADP+
SpSSADH prefers NADP+ over NAD+ as a hydride acceptor, structural basis of the cofactor preference of SpSSADH, overview. Residues Ser158 and Tyr188 in SpSSADH participate in the stabilization of the 2'-phosphate group of adenine-side ribose in NADP+
additional information
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NAD(P)+-dependent succinic semialdehyde dehydrogenase, BsSSADH shows similar values of the catalytic efficiency (kcat/Km) in both NAD+ and NADP+ as cofactors. The affinity of enzyme BsSSADH to NAD+ is approximately 2fold higher than that to NADP+, but the values of kcat are almost 2fold higher in the presence of NADP+ than NAD+
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additional information
although the binding affinity of cce4228 protein with NAD+ is about 50fold lower than that of cce4228 with NADP+, the catalytic efficiency of cce4228 protein towards succinic semialdehyde with saturated concentration of NADP+ is same as that with saturated concentration of NAD+ as its cofactors. Protein cce4228 harbors Ser157 and Lys154 residues in its active center which might determine the cofactor preference
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additional information
the Km value for NADP+ is 26fold higher than that for NAD+. The Km value for SSA increases 3fold with NADP+ in comparison with NAD+
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additional information
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the Km value for NADP+ is 26fold higher than that for NAD+. The Km value for SSA increases 3fold with NADP+ in comparison with NAD+
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