1.2.1.13: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating), go to the full flat file.

Reaction

Synonyms

A2B2-GAPDH, A2B2-glyceraldehyde-3-phosphate dehydrogenase, A4 glyceraldehyde 3-phosphate dehydrogenase, A4-GAPDH, At1g12900, At3g26650, D-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate phosphorylating), GapA, GapA-1, GAPA1, GAPA2, GapB, GAPC, GAPD, GAPDH, GAPDH (A4), glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde phosphate dehydrogenase (nicotinamide adenine dinucleotide phosphate phosphorylating), glyceraldehyde-3-dehydrogenase, glyceraldehyde-3-P dehydrogenase, glyceraldehyde-3-phosphate dehhydrogenase (NADP+) (phoshphorylating), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), glyceraldehyde-P dehydrogenase, GraP-DH, NAD(P)-GAPDH, NADP(H)-glyceraldehyde-3-phosphate dehydrogenase, NADP+-dependent G-3-P dehydrogenase, NADP+-dependent gapB, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent GAPDH, NADP-dependent glyceradehdye-3-phosphate dehydrogenase, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP-dependent glyceraldehyde phosphate dehydrogenase, NADP-dependent glyceraldehydephosphate dehydrogenase, NADP-G3PDH, NADP-GAPDH, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADP-GPD, NADP-triose phosphate dehydrogenase, NADPH-D-GA3P, NADPH-glyceraldehyde-3-phosphate dehydrogenase, NAPD-linked glyceraldehyde-3-P dehydrogenase, Ov-GAPDH, phosphorylating GAP dehydrogenase, phosphorylating glyceraldehyde-3-phosphate dehydrogenase, photosynthetic GAPDH, SSO0528, triosephosphate dehydrogenase (NADP+)

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.13 glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
53	Activating Compound
81	AA Sequence
5	Application
1	CAS Registry Number
25	Cloned(Commentary)
121	Cofactor
12	Crystallization (Commentary)
15	Disease/ Diagnostics
4	Expression
16	General Information
1	General Stability
2	IC50 Value
24	Inhibitors
7	Ki Value [mM]
117	KM Value [mM]
45	Localization
1	Metals/Ions
75	Molecular Weight [Da]
31	Natural Substrates/ Products (Substrates)
135	Organism
2	Oxidation Stability
6	Pathway
153	PDB ID
16	pH Optimum
1	pH Range
2	pI Value
37	Protein Variants
36	Purification (Commentary)
2	Reaction
15	Reaction Type
114	Reference
52	Source Tissue
45	Specific Activity [micromol/min/mg]
2	Storage Stability
80	Substrates and Products (Substrate)
51	Subunits
122	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
15	Temperature Stability [°C]
41	Turnover Number [1/s]

Activating Compound

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Activating Compound on EC 1.2.1.13 - glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Activating Compound Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,3-diphosphoglycerate

2 entries

2-mercaptoethanol

Synechococcus elongatus PCC 7942 = FACHB-805

-

activation

288008

ATP

5 entries

Br-

Spinacia oleracea

-

0.6 M, maximal stimulation

287990

cholate

Phaseolus vulgaris

-

activation

288010

Cl-

Spinacia oleracea

-

1.0 M, maximal stimulation

287990

Cl3CCOO-

Spinacia oleracea

-

0.24 M, maximal stimulation

287990

ClO4-

Spinacia oleracea

-

0.08 M, maximal stimulation

287990

cysteine

Pisum sativum

-

required for maximal activity

288036

dithiothreitol

9 entries

DTT

Asterionella formosa

-

activates

743366

Ferredoxin

Hordeum vulgare

-

enzyme is present throughout the life cycle of the plants, inactive enzyme is converted during greening to an active state by light either via enzyme effectors or via the ferredoxin-thioredoxin system

288029

-

glutathione

Pisum sativum

-

required for maximal activity

288036

glycerate 1,3-bisphosphate

Spinacia oleracea

-

most effective on a molar basis in stimulating NADPH-activity of dark chloroplast extracts and purified enzyme

288030

GSH

Synechococcus elongatus PCC 7942 = FACHB-805

-

activates in the physiological concentration range 0.1-2 mM

288008

I-

Spinacia oleracea

-

0.35 M, maximal stimulation

287990

NADP+

3 entries

NADPH

3 entries

phosphate

Spinacia oleracea

-

stimulates

288001, 288030

SCN-

Spinacia oleracea

-

0.05 M, maximal stimulation

287990

thioredoxin

2 entries

additional information

10 entries

-

1,3-diphosphoglycerate

Spinacia oleracea

-

stimulates

288001

1,3-diphosphoglycerate

Spinacia oleracea

-

the role of the reduction of the enzyme in vivo is to increase its sensitivity towards the activator 1,3-bisphosphoglycerate. The actual activation and aggregation state of the enzyme in chloroplasts in light is regulated by the concentration of 1,3-diphosphoglycerate as activator in the stroma and its actual activity by the availability of 1,3-diphosphoglycerate as substrate

288032

ATP

Euglena gracilis

-

activation

287973

ATP

Phaseolus vulgaris

-

activation

288010

ATP

Spinacia oleracea

-

activation

288030, 288034

ATP

Spinacia oleracea

-

activation in absence of NAD+, no activation in presence of NAD+

288032

ATP

Tetradesmus obliquus

-

high concentrations required for stimulation, activation by ATP is unlikely of physiological significance

288012

dithiothreitol

Galdieria sulphuraria

Q93YH6

full activation with 50 mM dithiothreitol

689485

dithiothreitol

Phaseolus vulgaris

-

activation

288010

dithiothreitol

Spinacia oleracea

-

-

288032

dithiothreitol

Spinacia oleracea

-

the role of the reduction of the enzyme in vivo is to increase its sensitivity towards the activator 1,3-bisphosphoglycerate

288021

dithiothreitol

Spinacia oleracea

-

activation by dithiothreitol + NADPH

288025

dithiothreitol

Synechococcus elongatus PCC 7942 = FACHB-805

-

activation

288008

dithiothreitol

Tetradesmus obliquus

-

activation

288012

dithiothreitol

Tetradesmus obliquus

-

when ATP is also present, the induced enzyme is stabilized

288012

dithiothreitol

Tetradesmus obliquus

-

the rate of induction of NADH-dependent activity by dithiothreitol increases hyperbolically with respect to NADPH concentration, implying that NADPH has more than a stabilising role in the activating process

288016

NADP+

Asterionella formosa

-

activates

743366

NADP+

Spinacia oleracea

-

activation

288030, 288034

NADP+

Spinacia oleracea

-

activation in absence of NAD+, no activation in presence of NAD+

288032

NADPH

Phaseolus vulgaris

-

activation

288010

NADPH

Spinacia oleracea

-

activation

288030, 288034

NADPH

Spinacia oleracea

-

activation in absence of NAD+, no activation in presence of NAD+

288032

thioredoxin

Hordeum vulgare

-

enzyme is present throughout the life cycle of the plants, inactive enzyme is converted during greening to an active state by light either via enzyme effectors or via the ferredoxin-thioredoxin system

288029

thioredoxin

Spinacia oleracea

-

enzyme is activated by thioredoxin that is reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol

288021

additional information

Arthrospira platensis

-

the light-induced activation of GAPDH is not associated with the presence of ATP

685291

-

additional information

Hordeum vulgare

-

enzyme is present throughout the life cycle of the plants, inactive enzyme is converted during greening to an active state by light either via enzyme effectors or via the ferredoxin-thioredoxin system

288029

-

additional information

Hordeum vulgare

-

the enzyme requires a high ratio of substrate to phosphate for activation. Mg2+ inhibits light activation of enzymes in intact chloroplasts. The substrate:phosphate ratio in the chloroplast may modulate enzyme activation in the light

288020

-

additional information

Phaseolus vulgaris

-

activation being dependent on oligomer dissociating to give protomers. The activation/dissociation process may be brought about by nucleotide binding, by sulfur bridge reduction or modification and by depression of hydrophobic interactions

288010

-

additional information

Pisum sativum

-

light activation of the enzyme within leaf chloroplast appears to be modulated by mediators which are reduced by photosynthetic electron flow from the photosystem I reaction center

288009

-

additional information

Spinacia oleracea

-

effect of an activator on the enzyme is selectively enhanced by either dithiothreitol-reduced thioredoxin-f or a cosolvent, and is inhibited by spermine

288001

-

additional information

Spinacia oleracea

-

complete light activation at 25 W*m-2 photosynthetically active radiation

288031

-

additional information

Spinacia oleracea

-

addition of organic solvents miscible in water to the activation process enhance the specific activity, cosolvents added during catalysis lower the rate of substrate conversion

288004

-

additional information

Sulfurisphaera tokodaii

-

no influence on activity is observed on assaying of the enzyme with 5 mM substrate and 0.5 mM co-substrate with 1 mM ATP, ADP, AMP, D-glucose 6-phosphate, D-fructose 6-phosphate, galactose 1-phosphate, D-glucose 1-phosphate or coenzyme A in either the glycolytic or gluconeogenic reaction

725013

-

additional information

Zea mays

-

complete light activation at 100 W*m-2 photosynthetically active radiation

288031

-