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1.2.1.10: acetaldehyde dehydrogenase (acetylating)

This is an abbreviated version!
For detailed information about acetaldehyde dehydrogenase (acetylating), go to the full flat file.

Word Map on EC 1.2.1.10

Reaction

acetaldehyde
+
CoA
 +
NAD+
=
acetyl-CoA
 +
NADH
 +
H+

Synonyms

4-hydroxy-2-ketovalerate aldolase/acylating acetaldehyde dehydrogenase, ACDH, acetaldehyde dehydrogenase, Acetaldehyde dehydrogenase [acetylating], acetaldehyde-alcohol dehydrogenase, acetyl-CoA reductase, acylating acetaldehyde dehydrogenase, AdhE, ADHES77, aldehyde dehydrogenase, aldehyde dehydrogenase (acylating), aldehyde/alcohol dehydrogenase, ALDH, BAD, bi-functional alcohol/aldehyde dehydrogenase, bifunctional acetaldehyde-alcohol dehydrogenase, BphJ, CoA-acylating aldehyde dehydrogenase, CoA-dependent aldehyde dehydrogenase, coenzyme A linked aldehyde dehydrogenase, coenzyme A-acylating aldehyde dehydrogenase, DmpF, DmpFG, MhpF, More, NAD+/CoA-dependent aldehyde dehydrogenase, nonphosphorylating acylating aldehyde dehydrogenase, PduP, Teth514_0627, Teth514_1942, Tsac_0416, TTHB247

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.10 acetaldehyde dehydrogenase (acetylating)

Purification

Purification on EC 1.2.1.10 - acetaldehyde dehydrogenase (acetylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents
-
by affinity chromatography by means of a Ni-Sepharose column
from anaerobically grown cells, rod-shaped
-
native enzyme from chloroplast stroma by anion exchange chromatography and affinity chromatography, followed by ultracentrifugation
native enzyme from plasma membrane by ultracentrifugation, hydroxyapatite and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration. The enzyme cannot be solubilized from membrane with detergents, either 1% Triton X-100 or 1% sulfobetaine 3-12
-
Ni-NTA column chromatography
on DEAE and NAD+-linked affinity columns, combined with an ammonium sulfate fractionation step
-
partial
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
-
recombinant N-terminally His6-tagged AdhE from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
under anaerobic conditions
-