Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.19.6.1: nitrogenase (flavodoxin)

This is an abbreviated version!
For detailed information about nitrogenase (flavodoxin), go to the full flat file.

Word Map on EC 1.19.6.1

Reaction

4 reduced flavodoxin + 2 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized flavodoxin + 2 NH4+ +
H2
+ 16 ADP + 16 phosphate

Synonyms

FeP, FldA, NifF, nitrogenase, nitrogenase Fe protein, nitrogenase Fe-protein

ECTree

     1 Oxidoreductases
         1.19 Acting on reduced flavodoxin as donor
             1.19.6 With dinitrogen as acceptor
                1.19.6.1 nitrogenase (flavodoxin)

Engineering

Engineering on EC 1.19.6.1 - nitrogenase (flavodoxin)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alphaH195Q
-
site-directed mutagenesis, the mutant shows an altered electron transfer substrate specificity compared to the wild-type enzyme,overview
alphaQ191K
-
site-directed mutagenesis, the mutant shows reduced electron transfer activity compared to the wild-type enzyme, it reduces only H+, not N2, overview
Q191A/V70A
-
site-directed mutagenesis, the double mutation does result in significant reduction of 2-butyne, with the exclusive product being 2-cis-butene
V70A
-
site-directed mutagenesis, substitution of alpha-70Val by alanine results in an increased capacity for the reduction of the larger alkyne propyne
V70G
-
site-directed mutagenesis, the mutant MoFe protein variant shows an increased capacity for reduction of the terminal alkyne, 1-butyne, but no detectable reduction of the internal alkyne 2-butyne
V70I
-
site-directed mutagenesis, substitution by isoleucine at this position nearly eliminates the capacity for the reduction of acetylene
Q191A/V70A
-
site-directed mutagenesis, the double mutation does result in significant reduction of 2-butyne, with the exclusive product being 2-cis-butene
-
V70A
-
site-directed mutagenesis, substitution of alpha-70Val by alanine results in an increased capacity for the reduction of the larger alkyne propyne
-
V70G
-
site-directed mutagenesis, the mutant MoFe protein variant shows an increased capacity for reduction of the terminal alkyne, 1-butyne, but no detectable reduction of the internal alkyne 2-butyne
-
V70I
-
site-directed mutagenesis, substitution by isoleucine at this position nearly eliminates the capacity for the reduction of acetylene
-
additional information