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1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
 + 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

pH Stability

pH Stability on EC 1.18.6.1 - nitrogenase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
MoFe protein stable
440167
8.7
-
50% loss of activity after overnight dialysis at pH 8.7
440167
9.5
the enzyme is stable when incubated at pH 9.5. At higher pH values and under turnover conditions, the enzyme is slowly inactivated. Initially the enzyme is reversibly inhibited (about 90%) for substrate reduction at pH 9.5, yet in a second, slower process, the enzyme becomes irreversibly inactivated as measured by substrate reduction at the optimal pH of 7.8. Incubation of the enzyme alone at pH 9.5 shows only minimal activity loss even after 4 h
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