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1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
 + 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

Specific Activity

Specific Activity on EC 1.18.6.1 - nitrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.024
-
purified mutant H195Q enzyme, anaerobic atmosphere
0.027
-
reductant dithionite, whole cell assay
0.066
-
crude extract, 100% Ar atmosphere, H2 production
0.13
-
V70I mutant, substrate acetylene, in presence of C2H4
0.17
-
V70I mutant, substrate N2, in presence of NH3
0.2
-
wild-type enzyme, substrate acetylene, in presence of H2
0.235
-
substrate N2, purified Mo nitrogenase
0.26
-
substrate C2H2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1
0.27
-
mutant A175G, purified enzyme, substrate C2H2
0.35
-
substrate N2, iron-only nitrogenase with Fe protein to FeFe protein ratio of 40:1
0.43
-
purified Fe protein
0.6
-
wild-type enzyme, substrate N2, in presence of NH3
0.6 - 0.8
-
purified Fe protein
0.64
-
wild-type enzyme, substrate N2, in presence of H2
1.01
-
purified MoFe protein
1.07
-
purified MoFe protein
1.26
-
purified Fe protein
1.3
-
substrate H+, purified Mo nitrogenase
1.5 - 1.7
-
purified MoFe protein
1.52
-
wild-type, purified enzyme, substrate C2H2
1.7 - 2.2
-
purified Fe protein
1.94
-
V70I mutant, substrate N2, in presence of H2
2.01
-
purified Fe protein
2.02
-
wild-type enzyme, substrate argon, in presence of H2
2.082
-
purified component II
2.16
-
purified component I
2.2
about, micromol/min/mg MoFe protein, mutant enzyme, pH not specified in the publication, temperature not specified in the publication
2.27 - 2.28
-
purified enzyme, substrate acetylene
2.316
about, micromol/min/mg MoFe protein, wild-type enzyme, pH not specified in the publication, temperature not specified in the publication
2.34
-
V70I mutant, substrate argon, in presence of H2
additional information