1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Reaction

4 reduced ferredoxin + 8 H+ +

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases

         1.18 Acting on iron-sulfur proteins as donors

             1.18.6 With dinitrogen as acceptor

                1.18.6.1 nitrogenase

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Results	in table
12	Activating Compound
18192	AA Sequence
4	Application
1	CAS Registry Number
16	Cloned(Commentary)
70	Cofactor
16	Crystallization (Commentary)
24	General Information
2	General Stability
125	Inhibitors
5	Ki Value [mM]
33	KM Value [mM]
13	Localization
445	Metals/Ions
138	Molecular Weight [Da]
204	Natural Substrates/ Products (Substrates)
311	Organism
54	Oxidation Stability
7	Pathway
412	PDB ID
14	pH Optimum
3	pH Range
3	pH Stability
2	Posttranslational Modification
112	Protein Variants
40	Purification (Commentary)
214	Reaction
3	Reaction Type
246	Reference
2	Renatured (Commentary)
17	Source Tissue
86	Specific Activity [micromol/min/mg]
4	Storage Stability
591	Substrates and Products (Substrate)
148	Subunits
88	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
3	Temperature Range [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 1.18.6.1 - nitrogenase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O

2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate

2 entries

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

80 entries

hydrazine + reduced ferredoxin

2 NH3 + oxidized ferredoxin

Azotobacter vinelandii

P00459

-

742740

-

-

?

hydroxylamine + reduced ferredoxin

NH3 + H2O + oxidized ferredoxin

Azotobacter vinelandii

P00459

-

742740

-

-

?

N2 + 10 H+ + 8 e- + 16 ATP

2 NH4+ + H2 + 16 ADP + 16 phosphate

2 entries

N2 + 4 reduced ferredoxin

hydrazine + 4 oxidized ferredoxin

Azotobacter vinelandii

P00459

-

742740

-

-

?

N2 + 8 e- + 16 ATP + 8 H+

2 NH3 + H2 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

the enzyme is responsible for biological nitrogen fixation, the conversion of atmospheric N2 to NH3

689764

-

-

?

N2 + 8 e- + 8 H+ + 16 ATP

2 NH3 + H2 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

-

715263

-

-

ir

nitrite + 4 reduced ferredoxin + 5 H+

hydroxylamine + H2O + 4 oxidized ferredoxin

Azotobacter vinelandii

P00459

-

742740

-

-

?

nitrite + H+ + ATP + reduced ferredoxin

NH3 + 2 H2O + 12 ADP + 12 phosphate + oxidized ferredoxin

Azotobacter vinelandii

P00459

overall reaction

742740

-

-

?

reduced ferredoxin + H+ + N2 + ATP

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Klebsiella pneumoniae

-

-

687822, 687823

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

18 entries

additional information

?

-

12 entries

2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O

2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate

Anabaena cylindrica

-

-

440170

-

-

?

2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O

2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate

Azotobacter vinelandii

-

-

440183

-

440183

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Allochromatium vinosum

-

-

440142, 440143, 440144, 440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena cylindrica

-

-

440144, 440145, 440170

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena cylindrica

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena cylindrica

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena sp.

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Anabaena sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azospirillum brasilense

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azospirillum sp.

-

-

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter chroococcum

-

-

440143, 440144, 440145, 440146, 440150, 440166, 440167

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter chroococcum

-

biological N2 fixation

440186

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter sp.

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

-

440138, 440142, 440143, 440144, 440145, 440146, 440147, 440150, 440153, 440154, 440155, 440156, 440158, 440163, 440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

-

440174

-

440174

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

-

440175, 440178, 440179, 440180, 440181, 440182, 440183, 440184, 440187

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

biological N2 fixation

440134, 440186

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

biological N2 fixation

440188

-

440188

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii

P00459

overall reaction

742740

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Azotobacter vinelandii OP

-

-

440180

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Beggiatoa alba

-

-

440160

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Beggiatoa alba B18LD

-

-

440160

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Bradyrhizobium japonicum

-

-

440143, 440144, 440145, 440151

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Bradyrhizobium lupini

-

-

440143, 440144, 440145, 440165

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Cereibacter sphaeroides

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Cereibacter sphaeroides

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Chlorobium sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Chlorobium sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Chromatium sp.

-

-

440174

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Chromatium sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Chromatium sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Clostridium pasteurianum

-

-

440142, 440143, 440144, 440145, 440146, 440147, 440153, 440159, 440164, 440174, 440180

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Clostridium pasteurianum

-

biological N2 fixation

440134, 440186

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Clostridium pasteurianum

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Corynebacterium flavescens

-

-

440144, 440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Corynebacterium flavescens 301

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Cyanobacterium sp.

-

ferredoxin normally functions as immediate electron donor to nitrogenase, during iron starvation it is replaced by flavodoxin

440148

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Cyanobacterium sp.

-

regulation

440148

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Cyanobacterium sp.

-

biological N2 fixation

440148

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Desulfovibrio desulfuricans

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Desulfovibrio sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Desulfovibrio sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Escherichia coli

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Escherichia coli C-M 74

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Frankia sp.

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Frankia sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Frankia sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Gloeocapsa sp.

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Gloeothece sp.

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Gloeothece sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Gloeothece sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Klebsiella pneumoniae

-

-

440140, 440142, 440143, 440144, 440145, 440146, 440147, 440149, 440152, 440157, 440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Klebsiella pneumoniae

-

-

440174

-

440174

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Klebsiella pneumoniae

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Leptolyngbya boryana

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Leptolyngbya boryana

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Leptolyngbya boryana

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Nitrospirillum amazonense

-

-

440161

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Nitrospirillum amazonense Y1

-

-

440161

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Ornithopus sativus

-

-

440145

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Oscillatoria sp.

-

-

440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Paenibacillus polymyxa

-

-

440143, 440144, 440145, 440146, 440147, 440169

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Paenibacillus polymyxa

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Paenibacillus polymyxa

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium leguminosarum

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium leguminosarum

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium sp.

-

-

440145, 440162, 440166, 440174

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium sp. ORS 571

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhizobium sp. ORS 571

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodobacter capsulatus

-

-

440142, 440171, 440176, 440177

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodobacter capsulatus B10S

-

-

440176, 440177

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodopseudomonas sp.

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodopseudomonas sp.

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodospirillum rubrum

-

-

440142, 440143, 440144, 440145, 440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodospirillum rubrum

-

biological N2 fixation

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Rhodospirillum rubrum

-

ferredoxin is the immediate electron carrier to nitrogenase in all nitrogen-fixing organisms with the exception of Klebsiella pneumoniae, and possibly Azotobacter species, where only flavodoxin is effective in coupling electron flow to nitrogenase

440134

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Trichormus variabilis

-

-

440148, 440152, 440166

-

-

?

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O

8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate

Xanthobacter autotrophicus

-

-

440168

-

-

?

N2 + 10 H+ + 8 e- + 16 ATP

2 NH4+ + H2 + 16 ADP + 16 phosphate

Azotobacter vinelandii

-

-

745173

-

-

?

N2 + 10 H+ + 8 e- + 16 ATP

2 NH4+ + H2 + 16 ADP + 16 phosphate

Clostridium pasteurianum

-

-

745173

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii

-

-

744780

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii

-

-

658010, 659426

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii

P07328; P07329

-

745155

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii

-

biological nitrogen fixation

658040

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii

P00459

enzyme complex is responsible for the majority of biological nitrogen fixation

658483

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Azotobacter vinelandii DJ1310

-

-

658010

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Bradyrhizobium japonicum

-

reduction of atmospheric dinitrogen to ammonium

660287

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Dysgonomonas capnocytophagoides

-

-

745127

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Fischerella sp.

-

-

746480

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Gluconacetobacter diazotrophicus

-

biological nitrogen fixation

658372

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Gluconacetobacter diazotrophicus PAL 5

-

biological nitrogen fixation

658372

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Herbaspirillum seropedicae

-

-

658769

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Klebsiella oxytoca

-

-

745127

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Klebsiella pneumoniae

-

-

658901

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Klebsiella pneumoniae M5a1

-

-

658901

-

-

ir

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Lacrimispora aerotolerans

-

-

745127

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Methanococcus maripaludis

-

-

684881

-

-

?

reduced ferredoxin + H+ + N2 + ATP + H2O

oxidized ferredoxin + H2 + NH3 + ADP + phosphate

Stenotrophomonas sp. gx-44

-

-

745127

-

-

?

additional information

?

-

Azotobacter vinelandii

P00459

the activity of the enzyme complex is regulated by specific interactions, inducing conformational changes, between the complex components, overview

658483

-

-

?

additional information

?

-

Azotobacter vinelandii

-

nitrogenase catalyzes the biological reduction of N2 to ammonia as well as the two-electron reduction of the nonphysiological alkyne substrate, alkyne substrate interaction within the nitrogenase MoFe protein, overview, the addition of neither 2-butyne-1-ol nor 2-butyne-1,4-diol to the growth medium has any effect on the capacity of wild-type Azotobacter vinelandii to sustain diazotrophic growth

688191

-

-

?

additional information

?

-

Azotobacter vinelandii

-

nitrogenase catalyzes the nucleotide-dependent conversion of dinitrogen to ammonia at the iron-molybdenum cofactor center of its molybdenum-iron protein component. Mo and homocitrate can be loaded onto the Fe protein upon ATP hydrolysis. Mo may enter the Fe protein by attaching to the position that corresponds to the gamma-phosphate of ATP following the hydrolysis of ATP. Subsequently, the loaded Fe protein can deliver Mo and homocitrate to the NifEN-associated precursor and transform the precursor into a fully matured iron-molybdenum cofactor

714796

-

-

?

additional information

?

-

Azotobacter vinelandii

-

in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons

728690

-

-

?

additional information

?

-

Azotobacter vinelandii

P00459

nitrite and hydroxylamine are nitrogenase substrates. The proposed NO2- reduction intermediate hydroxylamine (NH2OH) is a nitrogenase substrate, reduction intermediates can be trapped, cf. EC 1.7.2.2

742740

-

-

?

additional information

?

-

Azotobacter vinelandii DJ1260

-

in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons

728690

-

-

?

additional information

?

-

Azotobacter vinelandii DJ995

-

nitrogenase catalyzes the biological reduction of N2 to ammonia as well as the two-electron reduction of the nonphysiological alkyne substrate, alkyne substrate interaction within the nitrogenase MoFe protein, overview, the addition of neither 2-butyne-1-ol nor 2-butyne-1,4-diol to the growth medium has any effect on the capacity of wild-type Azotobacter vinelandii to sustain diazotrophic growth

688191

-

-

?

additional information

?

-

Gluconacetobacter diazotrophicus

-

influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview

658372

-

-

?

additional information

?

-

Gluconacetobacter diazotrophicus PAL 5

-

influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview

658372

-

-

?

additional information

?

-

Herbaspirillum seropedicae

-

gene nifX and the contigous gene orf1 are essential for maximum nitrogen fixation under iron limitation and are probably involved in synthesis of nitrogenase iron or iron-molybdenum clusters

658769

-

-

?

additional information

?

-

Mesorhizobium loti

-

enzyme activity causes acetylene production

716483

-

-

?

additional information

?

-

Methanococcus maripaludis

-

both nifI1 and nifI2 are required for regulation in vivo

684881

-

-

?