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1.18.6.1: nitrogenase

This is an abbreviated version!
For detailed information about nitrogenase, go to the full flat file.

Word Map on EC 1.18.6.1

Reaction

4 reduced ferredoxin + 8 H+ +

N2
+ 16 ATP + 16 H2O = 4 oxidized ferredoxin +
H2
 + 2 NH3 + 16 ADP + 16 phosphate

Synonyms

ARA, dinitrogenase, EC 1.18.2.1, Kp2, Mo nitrogenase, Mo-dependent nitrogenase, Mo-Fe nitrogenase, Mo-nitrogenase, MoFe protein, MoFe-protein, molybdenum nitrogenase, molybdenum-containing nitrogenase, molybdenum-iron protein, molybdenum-nitrogenase, More, N2ase, nif, nif1, nif2, nifD, NifDK, NifH, NifH2, nifHDK, nifK, nifM, nitrogenase Fe protein, nitrogenase Fe-protein, nitrogenase FeVco, nitrogenase iron-protein, nitrogenase MoFe protein, nitrogenase MoFe-protein, nitrogenase molybdenum iron protein, nitrogenase molybdenum-iron protein, V-nitrogenase, vanadium nitrogenase, vanadium-nitrogenase, VnfDGK, VnfH

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.6 With dinitrogen as acceptor
                1.18.6.1 nitrogenase

Crystallization

Crystallization on EC 1.18.6.1 - nitrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis of the iron protein, and of the MoFe protein
crystallized in presence of approx. 5% w/v polyethylene glycol 6000 and 0.2-0.4 M MgCl2 under strictly anaerobic conditions, x-ray analysis
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mutant enzyme R96Q bound to acetylene, capillary batch diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.1 M Tris (pH 8.0), 0.17 M Na2MoO4, and 0.001 M sodium dithionite
nitrogenase containing alpha70Ile mutant MoFe protein, 38 mg/ml protein is diluted in 50 mM Tris buffer, pH 8.0, and 250 mM NaCl, crystallization in 30% PEG 4000, 100 mM Tris, pH 8.0, 170-190 mM sodium molybdate, and 1 mM dithionite, 3-4 weeks, X-ray diffraction structure determination and analysis at 2.3 A resolution, comparison to the wild-type, with alpha70Val crystal structure, PDB ID 1M1N, overview
purified enzyme is diluted at room temperature with 3 volumes of Tris-HCl 0.01 M, pH 7.2, immediate crystal formation
-
sitting drop vapor diffusion method, using 150 mM ACES, 75 mM Tris, 75 mM ethanolamine (pH 9.8), 17-18% (w/v) PEG 3350, 0.8 M NaCl, and 1 mM sodium dithionite
sitting drop vapor diffusion method, using 23% polyethylene glycol 3350, 0.2 M lithium citrate, and 5 mM sodium dithionite
sitting drop vapor diffusion method, using 24-28% (w/v) PEG 8000, 0.75-0.85 M NaCl, 0.1 M imidazole/malate (pH 7.5), 1% glycerol (v/v), 0.5% 2,2,2-trifluoroethanol (v/v) and 2.5 mM Na2S2O4
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MoFe protein, crystallized in presence of approx. 5% w/v polyethylene glycol 6000 and 0.2-0.4 M MgCl2 under strictly anaerobic conditions, x-ray analysis
-
sitting drop vapor diffusion method, using 23% polyethylene glycol 3350, 0.2 M lithium citrate, and 5 mM sodium dithionite
purified recombinant His-tagged NifH2, hanging drop vapour diffusion method, 0.001 l of 20 mg/ml protein in 2 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.001 ml of reservoir solution, containing 0.1 M sodium citrate, 8% PEG 8000, pH 5.0, and equilibrated against 0.15 ml of reservoir solution, 10 days, 4°C, X-ray diffraction structure determination and analysis at 2.85 A resolution
-