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1.18.1.6: adrenodoxin-NADP+ reductase

This is an abbreviated version!
For detailed information about adrenodoxin-NADP+ reductase, go to the full flat file.

Word Map on EC 1.18.1.6

Reaction

2 reduced adrenodoxin +

NADP+
+
H+
= 2 oxidized adrenodoxin +
NADPH

Synonyms

AdR, adrenodoxin oxidoreductase, adrenodoxin reductase, ADX reductase, AdxR, FDXR, ferredoxin reductase, FLP, FprA, NADPH adrenodoxin reductase, NADPH-adrenodoxin reductase, NADPH-dependent adrenodoxin reductase, NADPH:adrenal ferredoxin adrenodoxin reductase, NADPH:adrenal ferredoxin oxidoreductase, NADPH:adrenodoxin oxidoreductase, NADPH:adrenodoxin oxidoreductase mitochondrial, NADPH:adrenodoxin reductase, nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase, TPNH-cytochrome P-450 reductase

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.1 With NAD+ or NADP+ as acceptor
                1.18.1.6 adrenodoxin-NADP+ reductase

Purification

Purification on EC 1.18.1.6 - adrenodoxin-NADP+ reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
adrenodoxin-Sepharose affinity column chromatography
-
ammonium sulfate precipitation, adrenodoxin-Sepharose 6B column chromatography and Sephadex G-100 gel filtration
-
DEAE-Fractogel column chromatography and adrenodoxin-Sepharose column chromatography
-
ion exchange chromatography and 2'-5'-ADP-Sepharose column chromatography
octylamino-Sepharose 4B column chromatography, Sephadex G- 100 gel filtration and DEAE-Biogel A column chromatography
-
recombinant enzyme from Escherichia coli to homogeneity
Sephadex G-100 gel filtration and DEAE-cellulose column chromatography
-
the tightly bound enzyme form is purified by sodium cholate solubilization, ammonium sulfate precipitation, DEAE-Sepharose column chromatography, 2’,5’-ADP-Sepharose column chromatography, and DEAE-Sepharose column chromatography. The loosely bound enzyme form is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, 2’,5’-ADP-Sepharose column chromatography, and Sephadex G-25 gel filtration
-