1.17.7.4: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase This is an abbreviated version! For detailed information about 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, go to the full flat file .
Reaction
dimethylallyl diphosphate +
2 oxidized ferredoxin [iron-sulfur] cluster
+
H2O =
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate +
2 reduced ferredoxin [iron-sulfur] cluster
+
2 H+
Synonyms (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1, 4-hydroxy-3-methylbut-2-enyl pyrophosphate reductase, BgHDR1, BgHDR2, chloroplast biogenesis6, Clb6, DsHDR, E-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, EC 1.17.1.2, HDR, HDR1, HMBPP reductase, HsHDR1, HsHDR2, isoprenoid synthesis H, ispH, lysis-tolerant B, LytB, reductase, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate, SrHDR
ECTree
Reaction
Reaction on EC 1.17.7.4 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
reaction mechanism, detailed overview
-
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
reaction mechanism, role of protein residues in the IspH mechanism, detailed overview
-
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
results show a bioorganometallic mechanism of IspH catalysis. There are two paramagnetic reaction intermediates proposed in this mechanism. The intermediate trapped with IspH E126Q mutants represents the 3-CH2OH-rotated weak pi-complex, intermediate II. Following a formally two-electron reduction and dehydration of the substrate, intermediate II is converted into an eta3-allyl complex with an oxidized HiPIP-like cluster [4Fe-4S]3+, intermediate III, which is observed with wild type IspH
-
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
-
-
-
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
reaction mechanism
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
Forms part of an alternative, nonmevalonate pathway for terpenoid biosynthesis. The enzyme acts in the reverse direction producing a 5:1 mixture of isopentenyl diphosphate and dimethyallyl diphosphate
-
-
-
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
reaction mechanism, detailed overview
-
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
reaction mechanism, role of protein residues in the IspH mechanism, detailed overview
-