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1.17.7.4: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

This is an abbreviated version!
For detailed information about 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, go to the full flat file.

Word Map on EC 1.17.7.4

Reaction

dimethylallyl diphosphate
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O
=
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate
+ 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+

Synonyms

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1, 4-hydroxy-3-methylbut-2-enyl pyrophosphate reductase, BgHDR1, BgHDR2, chloroplast biogenesis6, Clb6, DsHDR, E-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, EC 1.17.1.2, HDR, HDR1, HMBPP reductase, HsHDR1, HsHDR2, isoprenoid synthesis H, ispH, lysis-tolerant B, LytB, reductase, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate, SrHDR

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.7 With an iron-sulfur protein as acceptor
                1.17.7.4 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

Purification

Purification on EC 1.17.7.4 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified by using Strep-tactin chromatography
-
recombinant His-tagged apoprotein from Escherichia coli strain M15, to homogeneity
-
recombinant His-tagged IspH from strain XL-1 Blue by nickel affinity and anion exchange chromatography followed by dialysis, over 99% purity
-
recombinant His-tagged protein from Escherichia coli strain Xl1-Blue, to 95% homogeneity
recombinant His-tagged protein from Escherichia coli strain XL1-Blue, to homogeneity
using Ni-NTA chromatography
using Ni-NTA chromatpgraphy
-