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1.17.7.4: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

This is an abbreviated version!
For detailed information about 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, go to the full flat file.

Word Map on EC 1.17.7.4

Reaction

dimethylallyl diphosphate
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O
=
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate
 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+

Synonyms

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1, 4-hydroxy-3-methylbut-2-enyl pyrophosphate reductase, BgHDR1, BgHDR2, chloroplast biogenesis6, Clb6, DsHDR, E-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, EC 1.17.1.2, HDR, HDR1, HMBPP reductase, HsHDR1, HsHDR2, isoprenoid synthesis H, ispH, lysis-tolerant B, LytB, reductase, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate, SrHDR

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.7 With an iron-sulfur protein as acceptor
                1.17.7.4 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

Cloned

Cloned on EC 1.17.7.4 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from genomic DNA and expression as His-tagged protein in Escherichia coli strain XL-1 Blue
-
expressed as a Strep-tagged fusion protein in Escherichia coli
-
expressed in a Escherichia coli mutant strain MG1655 ara<>ispH
expressed in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli DH5alpha cells
expression in Escherichia coli
-
gene HDR, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional complementation by SmHDR1 of the Escherichia coli HDR mutant MG1655 ara/ispH, overexpression of SmHDR1 accelerates the biosynthesis of beta-carotene and enhances the production of tanshinones in cultured hairy roots of Salvia miltiorrhiza Bge. f. alba, transformation using Agrobacterium rhizogenes strain ACCC10060
gene HsHDR1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene HsHDR2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene ispH, expression as His-tagged protein in Escherichia coli strain XL1-Blue, construction of a synthetic operon containing ispH, ispG and gepE
gene ispH, the IspH domain of the Arabidopsis thaliana enzyme HDR corresponding to the full-length IspH of Escherichia coli fails to complement the Escherichia coli ispH mutant
gene lytB, expression as His-tagged enzyme in Escherichia coli strain XL1-Blue