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1.17.7.1: (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)

This is an abbreviated version!
For detailed information about (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin), go to the full flat file.

Word Map on EC 1.17.7.1

Reaction

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate
+
H2O
+
oxidized ferredoxin
=
2-C-methyl-D-erythritol 2,4-cyclodiphosphate
+
reduced ferredoxin
+ 2 H+

Synonyms

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, EC 1.17.4.3, GcpE, gcpE gene product, GcpE/IspG, IspG, IspG protein

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.7 With an iron-sulfur protein as acceptor
                1.17.7.1 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)

Purification

Purification on EC 1.17.7.1 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ferredoxin)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration
-
recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein
Thermosynechococcus vestitus