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1.17.4.4: vitamin-K-epoxide reductase (warfarin-sensitive)

This is an abbreviated version!
For detailed information about vitamin-K-epoxide reductase (warfarin-sensitive), go to the full flat file.

Word Map on EC 1.17.4.4

Reaction

phylloquinol
+
a protein with a disulfide bond
=
phylloquinone
 +
a protein with reduced L-cysteine residues

Synonyms

EC 1.1.4.1, phylloquinone epoxide reductase, reductase, phylloquinone epoxide, vitamin K 2,3-epoxide reductase, vitamin K 2,3-epoxide reductase complex subunit 1, vitamin K 2,3-epoxide reductase complex subunit-1, vitamin K epoxid reductase, vitamin K epoxide reductase, vitamin K epoxide reductase complex subunit 1, vitamin K oxidoreductase, Vitamin K reductase, vitamin K-2,3-epoxide reductase, vitamin K-2,3-epoxide reductase subunit 1, vitamin K-epoxide reductase, vitamin K1 epoxide reductase, vitaminK epoxide reductase, VKOR, VKOR complex, VKORC1, VKORC1 variant 2, VKORC1-like 1, VKORC1L1, VKORC1v2, warfarin sensitive vitamin K 2,3-epoxide reductase, warfarin-sensitive vitamin K1 2,3-epoxide reductase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.4 With a disulfide as acceptor
                1.17.4.4 vitamin-K-epoxide reductase (warfarin-sensitive)

Cloned

Cloned on EC 1.17.4.4 - vitamin-K-epoxide reductase (warfarin-sensitive)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of human coagulation factor (F)IX and c-myc-tagged VKORC1 mutant variants in HEK-293T cells in presence of varying warfarin concentrations
coexpression of human coagulation factor (F)IX andc-myc-tagged VKORC1 wild-type and mutants in HEK-293T cells in presence of varying warfarin concentrations
expressed in HEK-293 cells
expressed in Sf9 insect cells
-
expression in Actinomyces oris VKOR deletion (DELTAVKOR) mutant
-
expression in neural cell lines SK-N-MC and Neuro-2a, and in a human kidney cell line, HEK-293
expression in Pichia pastoris
expression in Pichia pastoris strain SMD 1168 Kex1-
-
expression in Sf9 cell
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expression in Sf9 cells
expression in Spodoptera frugiperda Sf21 cell microsomes using baculovirus containing wild-type or mutant VKORs transfection method, in vitro transcription and translation of the human enzyme using r-VKORC1/ZEM229 as the template
-
expression of c-myc-tagged enzyme mutants in Pichia pastoris membranes
expression of different constructs of N- or C-terminally NST-tagged or HPC4-tagged enzyme in insect cells, the NST tag is an N-linked glycosylation tag without a flexible linker, ER membrane topology of the recombinant enzymes, the tagged enzyme is glycosylated, overview
-
expression of mutant enzymes in Sf9 insect cells
expression of the human enzyme with the human herpesvirus 8 viral interleukin-6 in the yeast two-hybrid system using Saccharomyces cerevisiae strain AH109
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expressionof N- and C-terminally GFP-tagged enzyme in HEK293 cells, the N-terminus of VKOR resides in the ER lumen, whereas its C-terminus is in the cytoplasm
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gene rs9923231 or VKORC1, genotyping of rs9923231, quantitative RT-PCR expression analysis
gene rs9923231, quantitative reverse transcriptase–PCR expression analysis
gene rVkorc1, expression of C-terminally c-myc-tagged wild-type and mutant enzymes in Pichia patoris strain SMD1168 as membrane-bound proteins
gene Vkor, sequence comparisons
-
gene VKORC1 Y139F, DNA and amino acid sequence determination, genotyping, semiquantitative expression analysis
gene VKORC1, DNA and amino acid sequence determination, genotyping, semiquantitative expression analysis
gene VKORC1, expression of the c-myc-tagged VKORC1 in HEK-293 cells and in BHK21 cells
-
gene Vkorc1, expression of wild-type and mutant enzymes in Pichia pastoris as membrane bound protein
-
gene VKORC1, genotyping
gene VKORC1, genotyping, phenotypes of enzyme single nucleotide polymorphisms naturally occuring in Caucasian population
-
gene VKORC1, located on chromosome 1, DNA and amino acid sequenc determination and analysis, expression of wild-type and warfarin-resistant mutant enzymes in HEK-293 cells, expression of the latter alomost completely abolishes the enzyme activity
-
gene VKORC1, located on chromosome 16, DNA and amino acid sequenc determination and analysis, genetic structure, expression in Spodoptera frugiperda Sf9 cells and in Pichia pastoris
-
gene VKORC1, located on chromosome 7, DNA and amino acid sequenc determination and analysis
-
gene VKORC1, rapid genotyping for C1173T polymorphism, method evaluation
-
gene VKORC1, stable overexpression in factor IX BHK cell microsomes increasing 2.2fold the extent of secreted factor IX in vivo carboxylation, overview
-
genetic selection for mutants of Escherichia coli that promote the efficient membrane integration and expression of the mammalian membrane protein vitamin K epoxide reductase (VKORc1), that is ordinarily barely expressed in a wild-type Escherichia coli. When this poorly expressed enzyme is mutagenized or introduced to a library of Escherichia coli mutants, functional expression is achieved. The results suggest that YidC may play a role in quality control of membrane proteins at the level of insertion. Alteration of this function can greatly enhance functional overexpression of other membrane proteins
mutant enzymes C50A and C212A are both expressed in a Escherichia coli BL21(DE3)
overexpression of the enzyme in HEK-293 cell FX co-expressing factor X mutant I16L possessing a prothrombin propeptide
-
overexpression of the enzyme in HEK-293 cell FX co-expressing factor X mutant I16L possessing a prothrombin propeptide, expression analysis of the functional enzyme, overview
-
overexpression of VKOR in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
-
overexpression of wild-type and mutant enzymes in HEK293 cells
recombinant enzyme expression in Pichia pastoris strain SMD 1168 Kex1- endoplasmic reticulum membranes from a vector encoding (in 5'- to 3'-order): N-terminal alpha-mating factor from Saccharomyces cerevisiae, flag-tag epitope, His10-tag, Tev protease cleavage site, multiple cloning site, C-terminal Tev cleavage site, EGFP, Strep-tag II. The human VKORC1 DNA sequence is inserted into the multiple cloning site
-