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1.17.2.2: lupanine 17-hydroxylase (cytochrome c)

This is an abbreviated version!
For detailed information about lupanine 17-hydroxylase (cytochrome c), go to the full flat file.

Word Map on EC 1.17.2.2

Reaction

lupanine
+
2 ferricytochrome c
 +
H2O
=
17-hydroxylupanine
 + 2 ferrocytochrome c + 2 H+

Synonyms

lupanine dehydrogenase (cytochrome c), lupanine hydroxylase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.2 With a cytochrome as acceptor
                1.17.2.2 lupanine 17-hydroxylase (cytochrome c)

Renatured

Renatured on EC 1.17.2.2 - lupanine 17-hydroxylase (cytochrome c)

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, procedure is developed to renature and reactivate the enzyme, which is associated with the inclusion bodies. Reactivation requires addition of pyrroloquinoline quinone and is dependent on Ca2+
heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme