1.17.1.5: nicotinate dehydrogenase
This is an abbreviated version!
For detailed information about nicotinate dehydrogenase, go to the full flat file.
Word Map on EC 1.17.1.5
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1.17.1.5
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testosteroni
-
comamonas
-
putida
-
6-hydroxynicotinate
-
eubacterium
-
barkeri
-
multi-subunit
-
t7-like
-
pastoris
-
surpass
-
molybdenum
-
chassis
-
3-cyanopyridine
-
pichia
- 1.17.1.5
- testosteroni
-
comamonas
- putida
- 6-hydroxynicotinate
- eubacterium
- barkeri
-
multi-subunit
-
t7-like
- pastoris
-
surpass
- molybdenum
-
chassis
- 3-cyanopyridine
-
pichia
Reaction
Synonyms
dehydrogenase, nicotinate, EC 1.5.1.13, NA hydroxylase, NAH, NahAB1B2, NDH, NdhL, NdhM, NdhS, nicotinate dehydrogenase, nicotinic acid hydroxylase, three-component NA hydroxylase, three-component nicotinic acid hydroxylase
ECTree
1.17.1.5 nicotinate dehydrogenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.17.1.5 - nicotinate dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-pyrazinedicarboxylate + H2O + NADP+
?
-
10-20% of the activity with nicotinate
-
-
?
2-hydroxynicotinate + H2O + electron acceptor
?
-
hydroxylation at a low rate
-
-
?
3,5-pyridinedicarboxylate + H2O + NADP+
?
-
5-10% of the activity with nicotinate
-
-
?
3-cyanopyridine + H2O + electron acceptor
?
-
hydroxylation at a low rate
-
-
?
6-methylnicotinate + H2O + NADP+
?
-
5-10% of the activity with nicotinate
-
-
?
nicotinate + H2O + 2,6-dichloroindophenol
6-hydroxynicotinate + reduced 2,6-dichloroindophenol
nicotinate + H2O + phenazine methosulfate
6-hydroxynicotinate + reduced phenazine methosulfate
picolinic acid + NADP+ + H2O
6-hydroxypicolinic acid + NADPH + H+
-
-
-
-
?
trigonelline + H2O + NADP+
?
-
5-10% of the activity with nicotinate
-
-
?
2-pyrazinecarboxylate + H2O + NADP+
?
-
30% of the activity with nicotinate
-
-
r
2-pyrazinecarboxylate + H2O + NADP+
?
-
equally as effective as nicotinate
-
-
?
3-cyanopyridine + H2O + NADP+
6-hydroxy-3-cyanopyridine + NADPH + H+
-
-
-
?
3-cyanopyridine + H2O + NADP+
6-hydroxy-3-cyanopyridine + NADPH + H+
-
-
-
?
nicotinate + H2O + 2,6-dichloroindophenol
6-hydroxynicotinate + reduced 2,6-dichloroindophenol
Pusillimonas sp. (ex Stolz et al. 2005)
-
-
-
-
?
nicotinate + H2O + 2,6-dichloroindophenol
6-hydroxynicotinate + reduced 2,6-dichloroindophenol
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
-
-
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
flavin may be the primary electron acceptor, tetrazolium dye as electron acceptor
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
first step of nicotinate catabolism
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
-
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
equilibrium lies far in direction of 6-hydroxynicotinate formation
-
r
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
high substrate specificity, transfers electrons only to artificial electron acceptors of high redox potential, i.e. thiazolyl blue, nitroblue tetrazolium chloride, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol, methylene blue with 40% of the activity with phenazine ethosulfate and 2,6-dichlorophenolindophenol
-
r
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
first step of nicotinate catabolism
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
nicotinate degradation
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
nicotinate degradation
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
purified enzyme probably loses its own native electron acceptor during the course of solubilization and purification, electron acceptors: phenazine methosulfate, potassium ferricyanide and with lower activity nitro blue tetrazolium, negative polarity at the 3-position on the pyridine ring of the substrate is important for reaction
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
first step of nicotinate catabolism
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
purified enzyme probably loses its own native electron acceptor during the course of solubilization and purification, electron acceptors: phenazine methosulfate, potassium ferricyanide and with lower activity nitro blue tetrazolium, negative polarity at the 3-position on the pyridine ring of the substrate is important for reaction
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
first step of nicotinate catabolism
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
enzyme is linked to the cytochrome respiratory chain, phenazine methosulfate may accelerate the electron transfer from the electron respiratory chain to oxygen
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
-
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
-
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
-
-
?
nicotinate + H2O + electron acceptor
6-hydroxynicotinate + reduced electron acceptor
-
-
-
?
nicotinate + H2O + NAD+
6-hydroxynicotinate + NADH + H+
Pusillimonas sp. (ex Stolz et al. 2005)
-
-
-
-
?
nicotinate + H2O + NAD+
6-hydroxynicotinate + NADH + H+
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
-
-
-
?
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH
-
specific for NADP+, but artificial electron acceptors, i.e. benzyl viologen and 2,3,5-triphenyltetrazolium dyes, can replace NADP+, high substrate specificity with respect to nicotinate, requiring an unsubstituted pyridine or pyrazine ring nitrogen and a carboxyl group meta to the nitrogen
-
r
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH
-
equilibrium lies far in direction of 6-hydroxynicotinate formation
-
r
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH
-
first step in the anaerobic decomposition of nicotinate, NADP+ may be the natural, primary electron acceptor
-
?
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH
-
high substrate specificity toward electron donor substrates, unsubstituted nitrogen and a carboxyl group at position 3 are absolutely required for substrate hydroxylation and unsubstituted carbon-5 is important for oxidation at carbon-6 of substrate
-
?
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH
-
NADP+ or various dyes as electron acceptors
with NADPH as substrate, enzyme exhibits NADPH oxidase activity in presence of oxygen
r
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH + H+
-
-
-
?
nicotinate + H2O + NADP+
6-hydroxynicotinate + NADPH + H+
-
-
-
?
nicotinate + H2O + phenazine methosulfate
6-hydroxynicotinate + reduced phenazine methosulfate
-
-
-
?
nicotinate + H2O + phenazine methosulfate
6-hydroxynicotinate + reduced phenazine methosulfate
-
-
-
?
nicotinate + H2O + phenazine methosulfate
6-hydroxynicotinate + reduced phenazine methosulfate
Pusillimonas sp. (ex Stolz et al. 2005)
-
-
-
-
?
nicotinate + H2O + phenazine methosulfate
6-hydroxynicotinate + reduced phenazine methosulfate
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
-
-
-
?
pyridine-3-carboxylate + NADP+ + H2O
6-hydroxy-pyridine-3-carboxylate + NADPH + H+
-
-
-
-
?
pyridine-3-carboxylate + NADP+ + H2O
6-hydroxy-pyridine-3-carboxylate + NADPH + H+
-
-
-
-
?
additional information
?
-
-
not as substrates: nicotinamide, isonicotinate, picolinate, nicotinic acid N-oxide, N-methylnicotinamide, trigonelline, hypoxanthine, not as electron acceptor: NAD+
-
-
?
additional information
?
-
-
no activity with Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD(P)+, and both viologens as electron acceptors, not as substrates: 2- or 6-aminonicotinamide, 2-aminonicotinic acid, chelidamic acid, cinchomeronic acid, citrazinic acid, dinicotinic acid, dipicolinic acid, 3-hydroxypicolinic acid, hypoxanthine, isocinchomeronic acid, isonicotinic acid, lutidinic acid, nicotinamide, xanthine
-
-
?
additional information
?
-
-
not as substrates: nicotinamide, methyl nicotinate, beta-picoline, 3-pyridylcarbinol, quinolinic acid, 3-pyridylacetic acid, 3-hydroxypyridine, 3-chloropyridine, not as electron acceptors: methylene blue, FAD, FMN, NAD+, NADP+, horse heart cytochrome c, 2,3,5-triphenyl-2H-tetrazolium, neutral red, safranin O, menadione
-
-
?
additional information
?
-
-
not as substrates: nicotinamide, methyl nicotinate, beta-picoline, 3-pyridylcarbinol, quinolinic acid, 3-pyridylacetic acid, 3-hydroxypyridine, 3-chloropyridine, not as electron acceptors: methylene blue, FAD, FMN, NAD+, NADP+, horse heart cytochrome c, 2,3,5-triphenyl-2H-tetrazolium, neutral red, safranin O, menadione
-
-
?
additional information
?
-
Pusillimonas sp. (ex Stolz et al. 2005)
-
after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased
-
-
?
additional information
?
-
Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased
-
-
?
