1.17.1.5: nicotinate dehydrogenase

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For detailed information about nicotinate dehydrogenase, go to the full flat file.

Reaction

Synonyms

dehydrogenase, nicotinate, EC 1.5.1.13, NA hydroxylase, NAH, NahAB1B2, NDH, NdhL, NdhM, NdhS, nicotinate dehydrogenase, nicotinic acid hydroxylase, three-component NA hydroxylase, three-component nicotinic acid hydroxylase

ECTree

     1 Oxidoreductases

         1.17 Acting on CH or CH₂ groups

             1.17.1 With NAD⁺ or NADP⁺ as acceptor

                1.17.1.5 nicotinate dehydrogenase

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Results	in table
6	Activating Compound
1130	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
38	Cofactor
2	Crystallization (Commentary)
4	General Information
3	General Stability
22	Inhibitors
10	KM Value [mM]
5	Localization
2	Metals/Ions
25	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
29	Organism
2	Oxidation Stability
4	Pathway
11	pH Optimum
1	pH Range
3	pH Stability
4	Protein Variants
8	Purification (Commentary)
1	Reaction
9	Reaction Type
27	Reference
1	Renatured (Commentary)
2	Source Tissue
16	Specific Activity [micromol/min/mg]
3	Storage Stability
58	Substrates and Products (Substrate)
13	Subunits
25	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

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Cloned on EC 1.17.1.5 - nicotinate dehydrogenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Pseudomonas entomophila, subcloning of mutants in Escherichia coli failed

Pseudomonas putida

Q88FX8

696503

gene ndhL, belongs to the ndh cluster with genes ndhS and ndhM, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B

Comamonas testosteroni

B1PHD1, B1PHD2, B1PHD3

714382

gene ndhM, belongs to the ndh cluster with genes ndhL and ndhS, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B

Comamonas testosteroni

B1PHD1, B1PHD2, B1PHD3

714382

gene ndhS, belongs to the ndh cluster with genes ndhL and ndhM, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B

Comamonas testosteroni

B1PHD1, B1PHD2, B1PHD3

714382

genes nahAB1B2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme expression in the non-nicotinic acid-degrading Shinella sp. strain HZN7. The recombinant HZN7-pBBR-nahAB1B2 converts nicotinic acid into equimolar 6-hydroxynicotinic acid, while the recombinants HZN7-pBBR-nahAB1 (lacking component B2) and HZN7-pBBRnahAB2 (lacking component B1) cannot convert nicotinic acid. Cell-free extracts of HZN7-pBBR-nahAB1B2 exhibit nicotinic acid hydroxylase activity

Pusillimonas sp. (ex Stolz et al. 2005)

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745661