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1.17.1.4: xanthine dehydrogenase

This is an abbreviated version!
For detailed information about xanthine dehydrogenase, go to the full flat file.

Word Map on EC 1.17.1.4

Reaction

xanthine
+
NAD+
 +
H2O
=
Urate
 +
NADH
 +
H+

Synonyms

AtXDH1, EC 1.1.1.204, EC 1.2.1.37, IAO1, More, NAD-xanthine dehydrogenase, PaoABC, Retinol dehydrogenase, Rosy locus protein, VvXDH, xanthine dehydrogenase, xanthine dehydrogenase-1, xanthine dehydrogenase-2, xanthine dehydrogenase/oxidase, xanthine oxidoreductase, xanthine-NAD oxidoreductase, xanthine/NAD+ oxidoreductase, xanthine:NAD+ oxidoreductase, XDH, XDH/XO, XDH1, XDH2, XdhC, XOR, YagR, YagS, YagT

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.1 With NAD+ or NADP+ as acceptor
                1.17.1.4 xanthine dehydrogenase

Cloned

Cloned on EC 1.17.1.4 - xanthine dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminally His6-tagged wild-type and mutant XDHs expression in Escherichia coli strain TP1000
-
expressed in Escherichia coli
-
expression analysis
-
expression in Aspergillus nidulans
-
expression of enzyme mutant D428A in Spodoptera frugiperda Sf9 cells via the baculovirus transfection system in mostly the demolybdo-form
expression of His-tagged wild-type and mutant XDH1 variants in Pichia pastoris strain KM71
expression of His-tagged XDH1 in Pichia pastoris
expression of wild-type and mutant enzymes
gene VvXDH, sequence comparisons and phylogenetic analysis, synthesis of gene VvXDH through PCR-based two-step DNA synthesis method, recombinant overexpression in Arabidopsis thaliana ecotype Columbia under control of CaMV 35S promoter from plasmid pYM817 using the Agrobacterium tumefaciens strain GV3101 transfection method. The transgenic Arabidopsis thaliana plants show enhanced salt tolerance, phenotype, overview. Explicit function of VvXDH in conferring salt stress by increasing allantoin accumulation and activating abscisic acid signaling pathway, enhancing ROS scavenging in transgenic Arabidopsis thaliana. Expression of VvXDH results in elevated allantoin accumulation
gene Xdh, recombinant expression in Escherichia coli strain BL21(DE3), co-overexpression with three global regulators from Escherichia coli, IscS, TusA and NarJ and two Escherichia coli as well as two Rhodobacter capsulatus chaperone proteins, i.e. DsbA and DsbB, and NifS and XdhC (UniProt ID Q9X7K2), respectively, to aid the formation and ordered assembly of three redox center cofactors of Rhodobacter capsulatus XDH in Escherichia coli
gene xdh, sequence comparisons and phylogenetic analysis
gene xdh, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
gene xdh, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris
gene xdh, sequence comparisons and phylogenetic analysis, recombinant expression of liver XDH in insect cell system
-
gene xdh1, genotyping, map-based cloning of drf mutants, recombinant expression of wild-type and mutant enzymes in Pichia pastoris
gene XDH1, quantitative real-time PCR enzyme expression analysis
gene XDH1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
gene XDH2, quantitative real-time PCR enzyme expression analysis
genes xdhA-C, development of a method that translates active Rhodobacter capsulatus (alphabetagamma)2 XDH by directly expressing the iron-sulfur domain, the flavin adenine dinucleotide domain and the sulfurated molybdenum domain as three separate proteins in Escherichia coli, recombinant expression of wild-type enzyme and mutant enzymes, i.e. an (alphabetagamma)2 heterohexameric enzyme and two (alphabetagamma)2 XDH variants, in Escherichia coli
O54050; O54051; Q9X7K2
recombinant expression in Escherichia coli
-
recombinant expression of His-tagged enzyme in Escherichia coli strain TP1000
XOR is cloned and heterologously expressed in Spodoptera frugiperda insect cells
-