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1.16.3.1: ferroxidase

This is an abbreviated version!
For detailed information about ferroxidase, go to the full flat file.

Word Map on EC 1.16.3.1

Reaction

4 Fe(II) + 4 H+ +

O2
= 4 Fe(III) + 2 H2O

Synonyms

AfFtn, apoferritin, bacterial ferritin, bacterial ferroxidase, bacterioferritin, bacterioferritin B, BFR, BfrB, blue copper oxidase, caeruloplasmin, ceruloplasmin, Cp115, Cp135, Cp200, CT1740, CtFtn, cyto-FOX, cytosolic FOX, DdBfr, Dpr, Dps, Dps protein, Dps-like peroxide resistance protein, Dps-Te, DpsA, DpsA-Te, DspA, EncA, encapsulin, encapsulin A, ferritin, ferro-O2-oxidoreductase, ferro:O2 oxidoreductase, ferroxidase, ferroxidase center of bacterioferritin, ferroxidase I, ferroxidase II, ferroxidase, iron II:oxygen oxidoreductase, Fet3, FET3 gene product, fet3p, FOX1, Ftn, FtnA, H ferritin, H' ferritin, H-chain ferritin, Helicobacter pylori neutrophil-activating protein, hephaestin, HP-NAP, HuHF, human ceruloplasmin form I, human H ferritin, human H-chain ferritin, iron(II): oxygen oxidoreductase, L-ferritin, M ferritin, MaDps, MCO1, MmcO, mnxDEFG, monophenol-o-monoxygenase, More, mouse ceruloplasmin, multicopper ferroxidase, multicopper oxidase, multicopper oxidase 1, multicopper oxidase CueO, multicopper oxidase-1, mushroom tyrosinase, mycobacterial multicopper oxidase, neutrophil-activating protein, non-ceruloplasmin ferroxidase, non-specific DNA-binding protein Dps/ferroxidase, rhHp, rHuHF, Rv0846c, serum ferroxidase, VcDps, VCE_000308, xanthine oxidoreductase

ECTree

     1 Oxidoreductases
         1.16 Oxidizing metal ions
             1.16.3 With oxygen as acceptor
                1.16.3.1 ferroxidase

Purification

Purification on EC 1.16.3.1 - ferroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
after expression in baby hamster kidney cells
-
blood plasma ceruloplasmin was purified by DEAE-Sepharose chromatography
native enzyme from rat blood serum of untreated and Ag-treated animals by ion exchange chromatography and neomycin affinity chromatography
non-ceruloplasmin ferroxidase II
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recombinant ceruloplasmin, expressed in Pichia pastoris GS115 his4
recombinant enzyme from Escherichia coli
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recombinant Fet31 and Fet34 from Saccharomycs cerevisiae by glycosidase EndoH treatment and anion exchange chromatography
-
recombinant FLAG-tagged wild-type and mutants N113A and N194A, treatment with EndoH glycosidase
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged protein from Escherichia coli strain BL21(DE3) by heat treatment at 75°C for 10 min, dialysis, anion exchange chromatography, and affinity chromatography, followed by cleavage of the His-tag
Thermosynechococcus vestitus
recombinant N-terminally His-tagged wild-type and truncated mutant enzymes from Escherichia coli by immobilized metal affinity chromatography first under denaturing conditions and second as renaturation step by metal affinity chromatography under native conditions
recombinant soluble Fet3p
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 60°C for 10 min, followed by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
using a series of native chromatography steps: hydrophobic interaction, gel filtration, and ion exchange
-
using concanavalin-A-Sepharose and Q-sepharose
-
using NH4SO4 precipitation and gel filtration
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