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1.16.3.1: ferroxidase

This is an abbreviated version!
For detailed information about ferroxidase, go to the full flat file.

Word Map on EC 1.16.3.1

Reaction

4 Fe(II) + 4 H+ +

O2
= 4 Fe(III) + 2 H2O

Synonyms

AfFtn, apoferritin, bacterial ferritin, bacterial ferroxidase, bacterioferritin, bacterioferritin B, BFR, BfrB, blue copper oxidase, caeruloplasmin, ceruloplasmin, Cp115, Cp135, Cp200, CT1740, CtFtn, cyto-FOX, cytosolic FOX, DdBfr, Dpr, Dps, Dps protein, Dps-like peroxide resistance protein, Dps-Te, DpsA, DpsA-Te, DspA, EncA, encapsulin, encapsulin A, ferritin, ferro-O2-oxidoreductase, ferro:O2 oxidoreductase, ferroxidase, ferroxidase center of bacterioferritin, ferroxidase I, ferroxidase II, ferroxidase, iron II:oxygen oxidoreductase, Fet3, FET3 gene product, fet3p, FOX1, Ftn, FtnA, H ferritin, H' ferritin, H-chain ferritin, Helicobacter pylori neutrophil-activating protein, hephaestin, HP-NAP, HuHF, human ceruloplasmin form I, human H ferritin, human H-chain ferritin, iron(II): oxygen oxidoreductase, L-ferritin, M ferritin, MaDps, MCO1, MmcO, mnxDEFG, monophenol-o-monoxygenase, More, mouse ceruloplasmin, multicopper ferroxidase, multicopper oxidase, multicopper oxidase 1, multicopper oxidase CueO, multicopper oxidase-1, mushroom tyrosinase, mycobacterial multicopper oxidase, neutrophil-activating protein, non-ceruloplasmin ferroxidase, non-specific DNA-binding protein Dps/ferroxidase, rhHp, rHuHF, Rv0846c, serum ferroxidase, VcDps, VCE_000308, xanthine oxidoreductase

ECTree

     1 Oxidoreductases
         1.16 Oxidizing metal ions
             1.16.3 With oxygen as acceptor
                1.16.3.1 ferroxidase

Cloned

Cloned on EC 1.16.3.1 - ferroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clones encoding human CP identified, CP gene mapped to human chromosome 3q21-25 by human-mouse somatic-cell-hybrid analysis
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expressed in C6 glioma cells
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expressed in Escherichia coli
expressed in Escherichia coli to create mutants by site-directed mutagenesis
expressed in expressed in baby hamster kidney cells
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expression of FLAG-tagged wild-type and mutants N113A and N194A
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expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
FET3 gene cloned, strain M2 carrying plasmid pDY148 used as expression system, recombinant soluble Fet3p produced in yeast
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fully active recombinant human ceruloplasmin produced in the yeast Pichia pastoris
functional expression of Fet31 and Fet34 C-terminally fused to YFP or CFP in Saccharomyces cerevisiae enzyme ScFet3p-deficient strain plasma membrane, functional complementation. Other Fet proteins, e.g. CaFet33, from Candida albicans fail to complement the Saccharomyces cerevisiae mutant due to false localization at the vacuolar membrane
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gene ftn, sequence comparisons, recombinant expression of wild-type enzyme and mutant A19Y in Escherichia coli strain BL21(DE3)
gene Mco1, detailed sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes in Escherichia coli
gene sequencing and site-directed mutagenesis
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gene tll2470 or dpsA-Te, expression as soluble His-tagged protein in Escherichia coli strain BL21(DE3)
Thermosynechococcus vestitus
overexpressed via plasmid in Escherichia coli strain BL21-Gold (DE3)
recombinant expression in Escherichia coli
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recombinantly expressed in Sf9 cells using the baculovirus expression system. Recombinant MCO1 is expressed without the von Willebrand factor domains and transmembrane segment, and Arg454 is mutated to alanine to reduce proteolytic cleavage
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the mnxDEFG operon construct is expressed without a tag in Escherichia coli by inducing at 17°C and loading with 2 mM CuSO4 under microaerobic conditions
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