1.16.1.9: ferric-chelate reductase (NADPH)

This is an abbreviated version!
For detailed information about ferric-chelate reductase (NADPH), go to the full flat file.

Reaction

2 Fe(II)-siderophore +

Synonyms

FCR, FCR-LM, FCR-Os, Fe(III)-chelate reductase, ferredoxin-NADP(+) oxidoreductase, ferredoxin-NADP+ oxidoreductase, ferri-siderophore reductase, ferric chelate reductase, ferric reductase, ferric siderophore reductase, ferric-chelate reductase, FNR(S), FNRS, FRO1, FRO2, YqjH

ECTree

     1 Oxidoreductases

         1.16 Oxidizing metal ions

             1.16.1 With NAD⁺ or NADP⁺ as acceptor

                1.16.1.9 ferric-chelate reductase (NADPH)

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Results	in table
1	Activating Compound
642	AA Sequence
8	Cloned(Commentary)
16	Cofactor
1	Crystallization (Commentary)
6	Expression
12	General Information
16	kcat/KM [mM/s]
18	KM Value [mM]
4	Localization
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
39	Organism
1	pI Value
3	Protein Variants
5	Purification (Commentary)
1	Reaction
12	Reference
5	Source Tissue
4	Specific Activity [micromol/min/mg]
42	Substrates and Products (Substrate)
8	Subunits
30	Synonyms
1	Systematic Name
4	Turnover Number [1/s]

Purification

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Purification on EC 1.16.1.9 - ferric-chelate reductase (NADPH)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium sulfate precipitation, butyl Toyopearl column chromatography, DEAE Sepharose column chromatography, POROS HQ-H column chromatography, and Red Sepharose column chromatography

Synechocystis sp.

-

721789

HiTrap DEAE column chromatography, Superdex 75 gel filtration

Escherichia coli

-

722534

native enzyme

Synechocystis sp.

-

721789

Ni-NTA-Sepharose column chromatography and DEAE Sepharose column chromatography

Escherichia coli

-

721185

Strep-Tactin column chromatography

Escherichia coli

-

721629