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1.15.1.2: superoxide reductase

This is an abbreviated version!
For detailed information about superoxide reductase, go to the full flat file.

Word Map on EC 1.15.1.2

Reaction

superoxide
+
reduced rubredoxin
 + 2 H+ =
H2O2
 +
oxidized rubredoxin

Synonyms

1Fe SOR, 1Fe-SOR, 1Fe-superoxide reductase, 2Fe-SOR, class I SOR, class I superoxide reductase, class II SOR, cytochrome c–superoxide oxidoreductase, desulfoferrodoxin, desulforedoxin, Dfx, EC 1.18.96.1, Fe-SOR, GiSOR, MM_0632, More, neelaredoxin, neelaredoxin-type SOR, Nlr, PfSOR, rubredoxin oxidoreductase, SOR, superoxide reductase, TM0658, two-iron superoxide reductase, Zn/Fe-SOR

ECTree

     1 Oxidoreductases
         1.15 Acting on superoxide as acceptor
             1.15.1 Acting on superoxide as acceptor (only sub-subclass identified to date)
                1.15.1.2 superoxide reductase

Purification

Purification on EC 1.15.1.2 - superoxide reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
isolation of center I and II
Megalodesulfovibrio gigas
-
native and recombinant enzyme
-
recombinant Dfx from Escherichia coli strain BL21 by anion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme
recombinant enzyme from Escherichia coli by anion exchange chromatography, ultrafiltration, and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, anion exchange chromatography, and gel filtration
Megalodesulfovibrio gigas
-
recombinant enzyme with a fully oxidized metal center I
-
recombinant enzyme, gel filtration, anion exchange
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange and metal-chelate affinity chromatography
-
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, gel filtration and cleavage of the His-tag, followed by a second time nickel affinity chromatography and gel filtration
-
recombinant wild-type and mutant SORs, and rubredoxin from Escherichia coli strain BL21(DE3) to homogeneity by anion exchange chromatography and gel filtration
-
so far there is no suitable enzymatic assay for monitoring the purification from cell extracts
-
wild-type and E47A and K48A mutant
-
wild-type and E47A and K48I mutant enzyme
-