Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.99.22: ecdysone 20-monooxygenase

This is an abbreviated version!
For detailed information about ecdysone 20-monooxygenase, go to the full flat file.

Word Map on EC 1.14.99.22

Reaction

ecdysone
+
reduced acceptor
+
O2
=
20-hydroxyecdysone
+
acceptor
+
H2O

Synonyms

alpha-ecdysone C-20 hydroxylase, BmE20OHase, Cyp314A1, E-20-M, E20-M, E20M, E20MO, E20OHase, ecdysone 20-hydroxylase, ecdysone 20-monooxygenase, shade, shd

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.22 ecdysone 20-monooxygenase

Inhibitors

Inhibitors on EC 1.14.99.22 - ecdysone 20-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-anthraquinone
-
about 20% residual activity at 10 mM
1,9-Dideoxyforskolin
1H-[1,2,4]oxadizolo[4,3-a]-quinoxalin-1-one
-
a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 63% reduced E20M activity
2-isopropoxy-1,4-naphthoquinone
2-methoxy-1,4-anthraquinone
-
about 18% residual activity at 1 mM
2-methoxy-1,4-naphthoquinone
2-propoxy-1,4-naphthoquinone
20-hydroxyecdysone
6-anilinoquinoline-5,6-quinone
-
LY-38583, a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 80% reduced E20M activity, addition of guanylate cyclase activator S levels the activity to 78% of control activity
7-O-Hemisuccinyl-7-deacetyl-forskolin
9-hydroxy-1,4-anthraquinone
-
about 40% residual activity at 10 mM
9-methoxy-1,4-anthraquinone
-
about 45% residual activity at 10 mM
diquat dibromide
ecdysterone
-
competitive inhibition
forskolin
KCN
-
25% inhibition
KK-42
-
i.e. 1-benzyl-5[(E)-2,6-dimethyl-1,5-heptadienyl]imidazole
methylene blue
-
a guanylate cyclase inhibitor, applied with ecdysone agonist RH-5849 results in 86% reduced E20M activity, reversed by addition of dibutyryl-cGMP/theophylline to 87% of control activity
Metyrapone
NADP+
-
competitive inhibition
Non-ionic detergents
-
Tween 20, Tween 80 and Triton X-100
-
oxidized cytochrome c
-
reversed by KCN
-
quinizarin
-
about 60% residual activity at 10 mM
Tris/HCl-buffer
-
-
Tween 80
additional information
-