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1.14.99.22: ecdysone 20-monooxygenase

This is an abbreviated version!
For detailed information about ecdysone 20-monooxygenase, go to the full flat file.

Word Map on EC 1.14.99.22

Reaction

ecdysone
+
reduced acceptor
 +
O2
=
20-hydroxyecdysone
 +
acceptor
 +
H2O

Synonyms

alpha-ecdysone C-20 hydroxylase, BmE20OHase, Cyp314A1, E-20-M, E20-M, E20M, E20MO, E20OHase, ecdysone 20-hydroxylase, ecdysone 20-monooxygenase, shade, shd

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.22 ecdysone 20-monooxygenase

Cloned

Cloned on EC 1.14.99.22 - ecdysone 20-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein
-
cloned CYP6H1 cDNA is probably identical with purified enzyme
-
expressed in Sf9 insect cells
expression in S2 cells
gene SgShd, DNA and amino acid sequence determination and analysis
into a pSTBlue-1 Acceptor vector for sequencing, recombinant baculovirus harboring the BmE20OHase cDNA is constructed using the BAC-TO-BAC baculovirus expression system