1.14.99.15: 4-methoxybenzoate monooxygenase (O-demethylating)

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For detailed information about 4-methoxybenzoate monooxygenase (O-demethylating), go to the full flat file.

Word Map on EC 1.14.99.15

1.14.99.15

putida

rhodopseudomonas

palustris

heme

benzoic

ferredoxins

para-substituted

regioselectivity

o-demethylation

2-naphthoic

benzene

putidaredoxin

dioxygen

4-hydroxybenzoate

substrate-free

couplers

synthesis

Reaction

Synonyms

4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate monooxygenase, 4-methoxybenzoate O-demethylase, CYP199A2, CYP199A4, cytochrome P450 199A2, oxygenase, 4-methoxybenzoate 4-mono- (O-demethylating), p-anisic O-demethylase, piperonylate-4-O-demethylase, RPA1871

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.99 Miscellaneous

1.14.99.15 4-methoxybenzoate monooxygenase (O-demethylating)

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Results	in table
9	Activating Compound
25	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
26	Cofactor
3	Crystallization (Commentary)
11	General Information
6	General Stability
36	Inhibitors
37	KM Value [mM]
15	Metals/Ions
3	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
38	Organism
3	Oxidation Stability
2	Pathway
8	pH Optimum
1	pH Range
1	Posttranslational Modification
20	Protein Variants
8	Purification (Commentary)
4	Reaction
13	Reaction Type
21	Reference
55	Specific Activity [micromol/min/mg]
4	Storage Stability
127	Substrates and Products (Substrate)
4	Subunits
21	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Turnover Number [1/s]

KM Value

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KM Value on EC 1.14.99.15 - 4-methoxybenzoate monooxygenase (O-demethylating)

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0.0003 - 0.0007

3-Nitro-4-methoxybenzoate

2 entries

0.029

4-hydroxybenzoate

Pseudomonas putida

438348, 438349

0.00007 - 0.0014

4-Methoxybenzoate

2 entries

0.0058

4-methylaminobenzoate

Pseudomonas putida

438348, 438349

0.0062 - 0.009

4-Methylbenzoate

3 entries

0.0144 - 0.024

4-trifluoromethylbenzoate

3 entries

0.0367 - 0.055

benzoate

2 entries

0.077

N-methyl-4-aminobenzoate

Pseudomonas putida

438350, 438352

0.00063 - 0.008

NADH

2 entries

0.14

NADPH

Pseudomonas putida

reconstituted enzyme

438344, 438348

0.0019 - 0.055

6 entries

0.03

Putidamonooxin

Pseudomonas putida

438344

additional information

Rhodopseudomonas palustris

steady-state kinetics

688424