1.14.20.3: (5R)-carbapenem-3-carboxylate synthase

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For detailed information about (5R)-carbapenem-3-carboxylate synthase, go to the full flat file.

Word Map on EC 1.14.20.3

1.14.20.3

epimerization

desaturation

oxygenase

carbapenams

bicyclic

biosynthesized

donate

Reaction

Synonyms

carbapenem synthase, CarC

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.20.3 (5R)-carbapenem-3-carboxylate synthase

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Results	in table
1	Activating Compound
14	AA Sequence
3	Cloned(Commentary)
2	Crystallization (Commentary)
1	General Information
3	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
6	Pathway
3	PDB ID
1	pH Optimum
1	Purification (Commentary)
4	Reaction
9	Reference
10	Substrates and Products (Substrate)
1	Subunits
9	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 1.14.20.3 - (5R)-carbapenem-3-carboxylate synthase

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hanging-drop vapor diffusion method. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure is obtained with L-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems are used to investigate substrate binding

Pectobacterium carotovorum

Q9XB59

722630

structure of CarC/Fe(II)/2-oxoglutarate complex

Pectobacterium carotovorum subsp. carotovorum

Q9XB59

746493