1.14.20.1: deacetoxycephalosporin-C synthase

This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.

Word Map on EC 1.14.20.1

1.14.20.1

clavuligerus

chrysogenum

acremonium

isopenicillin

penicillium

beta-lactams

epimerase

cephamycins

cephalosporium

ring-expansion

7-aminodeacetoxycephalosporanic

pcbab

ironii

cephem

lactamdurans

synthesis

7-adca

2-oxoglutarate-dependent

cephalexin

carbenicillin

doacs

biotechnology

medicine

Reaction

Synonyms

acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.20.1 deacetoxycephalosporin-C synthase

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Results	in table
26	Activating Compound
24	AA Sequence
9	Application
1	CAS Registry Number
30	Cloned(Commentary)
24	Crystallization (Commentary)
18	General Information
4	General Stability
36	Inhibitors
33	kcat/KM [mM/s]
1	Ki Value [mM]
183	KM Value [mM]
3	Localization
26	Metals/Ions
26	Molecular Weight [Da]
75	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
73	Organism
4	Pathway
9	PDB ID
12	pH Optimum
7	pH Stability
3	pI Value
253	Protein Variants
29	Purification (Commentary)
6	Reaction
7	Reaction Type
57	Reference
1	Renatured (Commentary)
67	Specific Activity [micromol/min/mg]
3	Storage Stability
234	Substrates and Products (Substrate)
29	Subunits
66	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
135	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.14.20.1 - deacetoxycephalosporin-C synthase

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Amycolatopsis lactamdurans

60 min, stable

440378

Amycolatopsis lactamdurans

60 min, 25% loss of activity

440378

2 entries

Amycolatopsis lactamdurans

60 min, complete inactivation

440378

additional information

Acremonium chrysogenum

at pH 10 enzyme has similar stability at 4°C and at 25°C. At pH 11 the enzyme is more stable at 4°C than at 25°C

440375