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1.14.20.1: deacetoxycephalosporin-C synthase

This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.

Word Map on EC 1.14.20.1

Reaction

Penicillin N
+
2-oxoglutarate
 +
O2
=
deacetoxycephalosporin C
 +
succinate
 +
CO2
 +
H2O

Synonyms

acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.1 deacetoxycephalosporin-C synthase

Inhibitors

Inhibitors on EC 1.14.20.1 - deacetoxycephalosporin-C synthase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate
-
reversible, 0.04 mM, 90% inhibition
1,10-phenanthroline
-
-
2-oxobutyrate
-
0.1 mM, complete inhibition
3-exomethylenecephalosporin
-
strong, substrate for hydroxylation reaction of enzyme, EC 1.14.11.26
3-exomethylenecephalosporin C
-
0.28 mM, 71% inhibition of the formation of deacetoxycephalosporin C
3-oxoadipate
-
0.1 mM, 56% inhibition
5,5'-dithiobis-2-nitrobenzoic acid
ammonium hydrogencarbonate
-
100-500 mM, complete inactivation
ampicillin
-
2.8 mM, 13% inhibition of the formation of deacetoxycephalosporin C
bathophenanthroline
-
-
Cu2+
-
1 mM, complete inhibition
iodoacetic acid
N-ethylmaleimide
Ni2+
-
inhibition in decreasing order, Zn2+, Co2+, Ni2+
o-phenanthroline
p-hydroxymercuribenzoate
penicillin G
-
2.8 mM, 57% inhibition of the formation of deacetoxycephalosporin C
Penicillin V
-
2.8 mM, 47% inhibition of the formation of deacetoxycephalosporin C
additional information
-
when 2-oxoglutarate is added to the enzyme before the mixture of ATP, Fe2+ and ascobate activity is only about 40% of the reaction
-