Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.19.9: tryptophan 7-halogenase

This is an abbreviated version!
For detailed information about tryptophan 7-halogenase, go to the full flat file.

Word Map on EC 1.14.19.9

Reaction

tryptophan
+
FADH2
 +
chloride
 +
O2
 +
H+
=
7-chloro-L-tryptophan
 +
FAD
 + 2 H2O

Synonyms

EC 1.14.14.7, FAD-dependent halogenase, flavin-dependent halogenase, flavin-dependent tryptophan halogenase, halogenase, KtzQ, L-tryptophan-7-halogenase, PrnA, RebH, Trp 7-hal, Trp halogenase, Trp-7-Hal, Trp-7-halogenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.9 tryptophan 7-halogenase

Cloned

Cloned on EC 1.14.19.9 - tryptophan 7-halogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned by PCR and fused to the tac promoter from Escherichia coli
expressed in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3)
gene ktzQ, cloned from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression as soluble N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene rebH, functional recombinant expression of Yfp-tagged enzyme in Nicotianan benthamiana leaf cytosol via Agrobacterium tumefaciens tranfection method under control of the CaMV 35S promoter leading to formation of 7-chlorotryptophan, coexpression with gene rebF from Lechevalieria aerocolonigenes, different genetic constructs, overview. Coexpression and concerted activity of tryptophan 6-halogenase Stth, EC 1.14.19.59, and RebH lead to formation of 6,7-halotryptophan
gene RebH, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
mutant enzyme Y455W is expressed in Catharanthus roseus
recombinant expression in Escherichia coli