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the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
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the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
additional information
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the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
additional information
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the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
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additional information
following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 is grown in the field and produces a 70 molar per cent high oleic acid (18:1DELTA9) oil at yields similar to wild-type. Cold-pressed high oleic oil produces fewer volatiles and has a sevenfold increase in shelf life compared to wild-type. Two low abundance octadecadienoic acids, 18:2DELTA6,9 and 18:2DELTA9,15, are identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Dramatic fatty acid level changes observed in csfad2a-1 seed confirm that the predicted truncated CSFAD2A protein is nonfunctional. A second allele, csfad2a-2, which carries two point mutations giving rise to a proline to leucine transition at positions 218 and 375 of the predicted amino acid sequence of CSFAD2A. Homozygous csfad2a-2 (BC1F1) seed accumulate nearly 70 molar per cent of oleic acid, low levels of 18:2DELTA6,9 and 18:2DELTA9,15 and decreased levels of linoleic acid and alpha-linolenic acid compared to heterozygous and segregating wild-type seeds from the same population. This seed oil phenotype is very similar to that of csfad2a-1 and is consistent with one or both of the P to L transitions disrupting protein function
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a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
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a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
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a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
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for improvment of the linoleic acid content in the lipids of Rhodosporidium toruloides, galactose-inducible expression of the gene encoding DELTA12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of Rhodosporidium toruloides haploid and diploid strains
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a transgenic Saccharomyces cerevisiae strain BYdesa, expressing the enzyme from Helvea brasiliensis, produces up to 15% polyunsaturated fatty acids, mostly 9Z,12Z-C18:2, linoleic acid, but also 9Z,12Z-C16:2 fatty acid under inducing conditions, production of 4-hydroxy-2-nonenal, one of the major end products of n-6 polyunsaturated fatty acid peroxidation. Desaturase expression causes adaptation to oxidative stress but not to hyperosmotic stress, phenotype, overview
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the DELTA12 desaturase-defective mutant, Mut48, derived from Mortierella alpina 1S-4 produces several fatty acids of the n-9 family such as 6,9-octadecadienoic acid (18:2n-9), 8,11-eicosadienoic acid (20:2n-9), and mead acid. The mutants SR88 and TM912 exhibit a complete DELTA12 desaturation deficiency with no arachidonic acid accumulation
additional information
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for improvment of the linoleic acid content in the lipids of Rhodosporidium toruloides, galactose-inducible expression of the gene encoding DELTA12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of Rhodosporidium toruloides haploid and diploid strains
additional information
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the DELTA12 desaturase-defective mutant, Mut48, derived from Mortierella alpina 1S-4 produces several fatty acids of the n-9 family such as 6,9-octadecadienoic acid (18:2n-9), 8,11-eicosadienoic acid (20:2n-9), and mead acid. The mutants SR88 and TM912 exhibit a complete DELTA12 desaturation deficiency with no arachidonic acid accumulation
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additional information
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construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview
additional information
construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview