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1.14.19.6: acyl-CoA (9+3)-desaturase

This is an abbreviated version!
For detailed information about acyl-CoA (9+3)-desaturase, go to the full flat file.

Word Map on EC 1.14.19.6

Reaction

oleoyl-CoA
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
linoleoyl-CoA
 + 2 ferricytochrome b5 + 2 H2O

Synonyms

acyl-CoA (9+3)-desaturase, acyl-lipid DELTA12-desaturase, Cofad2-2, CSFAD2A, D12Des, DELTA 12 desaturase, delta 12 fatty acid desaturases, delta-12 fatty acid desaturase, delta-12 fatty acid desaturase 2, DELTA12 acyl-lipid desaturase, DELTA12 desaturase, DELTA12 FAD, DELTA12 fatty acid desaturase, DELTA12 oleate hydroxylase, DELTA12(omega6)-desaturase, DELTA12-acyl-lipid desaturase, DELTA12-desaturase, DELTA12-desaturase system, DELTA12-FAD, DELTA12-fatty acid desaturase, DELTA12-fatty acyl-CoA desaturase, DELTA12des, DELTA12DS, Des12, DesA, egFAD2, FAD-2, FAD2, FAD2-2, FAD2A, FADS1, FADS2, FAH, FAT-2, fatty acid desaturase-2, Fm2, KlFAD2, Le-FAD2, LKFAD15, microsomal Delta-12 fatty acid desaturase, microsomal delta-12 oleate desaturase, microsomal oleate desaturase, microsomal omega-6 fatty acid desaturase, oleoyl coenzyme A desaturase, oleoyl-CoA DELTA12 desaturase, oleoyl-DELTA12 desaturase, oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, omega-6 fatty acid desaturase, PtFAD2, PtFAD6, seed-specific delta-12 fatty acid desaturase 2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.6 acyl-CoA (9+3)-desaturase

Cloned

Cloned on EC 1.14.19.6 - acyl-CoA (9+3)-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, high level expression in Escherichia coli strains JM109 and BL21(DE3)
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expressed in Anacystis nidulans strain R2-SPc
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum cells
expressed in Mus musculus strains C57BL/6 and DBA/2
-
expressed in Nicotiana tabacum cultivar Wisconsin
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae and in Arabidopsis thaliana
expressed in Saccharomyces cerevisiae and in the Arabidopsis thaliana fad2-1 mutant (knockout mutants lacking the single FAD2 gene)
expressed in Saccharomyces cerevisiae EH1315, 7.9% oleic acid and 29% linoleic acid in yeast expressing DELTA12 desaturase compared with 37% oleic acid and no detectable linoleic acid in control yeast, fatty acid composition analyzed by gas-liquid chromatography
expressed in Saccharomyces cerevisiae INVSc1 cells and Aurantiochytrium limacinum mh0186 cells
-
expressed in Saccharomyces cerevisiae INVScI and Pichia pastoris GS115 cells
-
expressed in Saccharomyces cerevisiae strain IFO10150
-
expressed in Saccharomyces cerevisiae strain InvSc1
-
expressed in Solanum lycopersicum cultivar Desnitsa
expressed in Solanum tuberosum cultivar Desnitsa
expressed in wild-type Yarrowia lipolytica and its DELTA12 desaturase knockout mutant, 62.6 weight percent of total fatty acid was linoleic acid produced in the mutant
expression in Saccharomyces cerevisiae
-
expression in Saccharomyces cerevisiae leads to endogenous production of linoleic acid, the proportion of linoleic acid in the total fatty acids produced by transformed Saccharomyces cerevisiae increases from 1.1 mol% when grown at 30°C to 2.9 mol% when grown at 15°C
expression in Saccharomyces cerevisiae strain BYdesa
-
expression in Saccharomyces cerevisiae strain YHU3046-4A results in endogenous production of linoleic acid, increasement in production of linoleic acid from 0.66 to 1.19 microg/mg dry cell weight when 0.5 microM oleic acid as a substrate was exogenously added
-
expression of DELTA12-fatty acid desaturase genes in Escherichia coli strain BL21 and Saccharomyces cerevisiae strain IN-VSc1 leads to production of an active enzyme which converts 17.876% and 17.604% of oleic acid to linoleic acid, GC-MS detection in vitro and in vivo
functional expression of a DELTA12 fatty acid desaturase gene from Spinacia oleracea in transgenic Sus scrofa. Levels of linoleic acid (18:2n-6) in adipocytes that have differentiated in vitro from cells derived from the transgenic pigs are about 10times higher than those from wild-type pigs. In addition, the white adipose tissue of transgenic pigs contained about 20% more linoleic acid (18:2n-6) than that of wild-type pigs
gene An1, DNA and amino acid sequence determination and analysis, functional expression in Arabidopsis thaliana
gene An2, DNA and amino acid sequence determination and analysis, functional expression in Arabidopsis thaliana
gene Cofad2-2, DNA and amino acid sequence determination and analysis, sequence comparisons
gene Cop-odeA, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae strain EH1315
gene Cs-fad2, DNA and amino acid sequence determination and analysis, transcriptional analysis, and functional expression in and complementation of enzyme-deficient Saccharomyces cerevisiae S288C mutant strain NBRC1136
gene desA, recombinant expression of the desA-licBM3 hybrid in transgenic Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum plants
gene egFAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 leading to production of linoleic acids (18:2DELTA9,12), not normally present in wild-type yeast cells, evaluation of fatty acids profiles of the transformed yeast cells by GC/MS analysis, overview
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2A, quantitative RT-PCR enzyme expression analysis, CSFAD2A is cloned into the expression vector pESCTRP containing the galactose-inducible GAL1 promoter, recombinant expression in Saccharomyces cerevisiae
gene FAD6, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc1, expression analysis
gene Pc-fad2, DNA and amino acid sequence determination and analysis
gene Ssd12, DNA and amino acid sequence determination and analysis, two genomic copies, expression in Saccharomyces cerevisiae
heterologous expression in Saccharomyces cerevisiae and Aspergillus oryzae
heterologous expression in yeast Saccharomyces cerevisiae and Synechococcus
-
recombinant expression in Rhodosporidium toruloides with integration into the genome using Agrobacterium tumefaciens strain AGL1, the recombinnat expression leads to increased production of linoleic acid from oleic acid in the tranformed cells, while no significant decrease in the relative contents of the other fatty acids, namely, C14:0 (myristic acid), C16:0 (palmitic acid), and C16:1 (palmitoleic acid) is observed
recombinant expression of His6-tagged enzyme FADS1 in Rhodosporidium toruloides with integration into the genome using Agrobacterium tumefaciens strain AGL1, the recombinnat expression leads to increased production of linoleic acid from oleic acid in the tranformed cells, while no significant decrease in the relative contents of the other fatty acids, namely, C14:0 (myristic acid), C16:0 (palmitic acid), and C16:1 (palmitoleic acid) is observed
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