Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.19.5: acyl-CoA 11-(Z)-desaturase

This is an abbreviated version!
For detailed information about acyl-CoA 11-(Z)-desaturase, go to the full flat file.

Word Map on EC 1.14.19.5

Reaction

long-chain acyl-CoA
+
reduced acceptor
 +
O2
=
DELTA11-acyl-CoA
 +
acceptor
 + 2 H2O

Synonyms

(Z)-11 myristoyl CoA desaturase, acyl-CoA DELTA11-desaturase, acyl-CoA desaturase, acyl-CoA Z/E11 desaturase, APTQ desaturase, bifunctional DELTA11-desaturase, bifunctional Z-DELTA11-desaturase, Cro-Z/E11, CroDELTA11 desaturase, DELTA11 desaturase, DELTA11-(Z)-desaturase, DELTA11-desaturase, DELTA11-fatty-acid desaturase, DELTA11-myristoyl-CoA desaturase, DELTA11-palmitoyl-CoA-desaturase, DELTA11-palmitoyl-coenzyme A desaturase, desat1, desaturase, myristoly coenzyme A (E)-11, Dpu-DELTA11-APSQ, Dpu-DELTA11-LPAE, EC 1.14.99.32, fatty acid DELTA11-desaturase, fatty acyl DELTA11-desaturase, LATPG1, Lca-KPVQ, MambrD11-desaturase, More, myristoyl-CoA 11-(Z) desaturase, OfuZ/E11 protein, OscZ/E11 protein, PDesat-TnDELTA11 Z protein, Sls//E11, sphingolipid long chain base delta 8 desaturase, TpDESN, Z/E11-desaturase, Z/EDELTA11

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.5 acyl-CoA 11-(Z)-desaturase

Cloned

Cloned on EC 1.14.19.5 - acyl-CoA 11-(Z)-desaturase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminal His-tagged fusion protein expressed using recombinant baculoviruses
-
DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme as fusion protein with beta-actin from Mamestra brassicae, UniProt ID B0LF73, quantitative real-time PCR enzyme expression analysis
expressed as polyhistidine-tagged protein in elongase 1 and DELTA9 desaturase-deficient yeast cells
-
expressed in Sf9 insect cells
expressed in yeast
expression in Saccharomyces cerevisiae DELTAelo1/DELTAole mutant strain, which is both elongase 1 and DELTA9 desaturase-deficient, using a Cu+2 inducible expression vector, the recombinant expression leads to producing large quantities of C11-monounsaturated fatty acids, mainly (Z)-11-hexadecenoic acid, (E,E)-10,12-tetradecadienoic acid, minor amounts of (E,Z)-10,12-hexadecadienoic acid, and very low amounts of (E,Z)-10,12-tetradecadienoic isomer
-
expression in yeast
gene d2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of MsexAPTQ desaturase in Saccharomyces cerevisiae strain W303
genetic trans-formation of a desaturase-deficient strain of the yeast Sac-charomyces cerevisiae with an expression plasmid encoding PDesat-Tn DELTA11Z results in complementation of the strain's fatty acid auxotrophy and the production of DELTA11 Z-unsaturated fatty acids
heterologously expressed in Saccharomyces cerevisiae
-
into a copper-inducible pYEX vector to assess its desaturase activity and then transformed into a desaturase- and elongase-deficient mutant ole1 elo1 yeast strain
partial cDNAs are amplified and cloned into the vector pCold I DNA, recombinant proteins are produced by Escherichia coli BL21 cells and used as antigens to raise antibodies in mice
recombinant enzyme expression in Saccharomyces cerevisiae double deficient ole1 elo1 strain