1.14.19.33: DELTA12 acyl-lipid conjugase (11E,13E-forming)
This is an abbreviated version!
For detailed information about DELTA12 acyl-lipid conjugase (11E,13E-forming), go to the full flat file.
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 + 2 H2O
Synonyms
DELTA12 desaturase, DELTA12 oleate desaturase, FADX, FADX-1A, FADX-1B, FADX-2, fatty acid DELTA12-conjugase
ECTree
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Substrates Products
Substrates Products on EC 1.14.19.33 - DELTA12 acyl-lipid conjugase (11E,13E-forming)
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REACTION DIAGRAM
(6Z,9Z,12Z)-octadeca-6,9,12-trienoate + 2 ferrocytochrome b5 + O2 + 2 H+
(6Z,9Z,11E,13E)-octadeca-6,9,11,13-tetraenoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-hexadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12E)-hexadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
(9Z)-octadecenoate + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12E)-octadeca-9,12-dienoate + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-parinaroyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
the enzyme is involved in the biosynthetic pathway of eleostearic acid
alpha-parinarate = (9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate
-
?
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
the enzyme is involved in the biosynthetic pathway of eleostearic acid
alpha-eleostearate = (9Z,11E,13E)-octadeca-9,11,13-trienoate
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-eleostearoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
the double bond introduced by FADX during fatty acid desaturation is in the trans, rather than cis, configuration
-
-
?
(13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
0.7% conversion
-
-
?
octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
0.8% conversion
-
-
?
octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
18.7% conversion
-
-
?
octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
(13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
?
-
the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid
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-
?
additional information
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the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid
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?
additional information
?
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the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid
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?
additional information
?
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the wild-type enzyme produces predominantly conjugated alpha-eleostearic acid and little punicic acid from its substrate linoleic acid. The helix 2 and the first histidine box are a determinant of conjugase product partitioning into alpha-punicic acid ((9Z,11E,13E,15Z)-octadeca-9,11,13,15-tetraenoate) or alpha-eleostearic acid ((9Z,11E,13E)-octadeca-9,11,13-trienoate), sequence comparisons, overview. Residues 111 and 115 of the enzyme exhibit an interactive effect in punicic acid formation
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?
additional information
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linoleic acid serves as a precursor for punicic acid synthesis by the enzyme
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additional information
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the enzyme catalyzes the conversion of the DELTA12-double bond of linoleic acid (18:2DELTA9cis,12cis) into conjugated DELTA11trans and DELTA13cis-double bonds
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?
additional information
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the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid
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?
additional information
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the enzyme is capable of generating a variety of alternative conjugated and 12-desaturated fatty acid products in yeast cells cultured in the presence of exogenously supplied fatty acid substrates
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additional information
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a bifunctional enzyme also exhibiting the activity of EC 1.14.19.34
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?