1.14.19.29: sphingolipid 8-(E/Z)-desaturase
This is an abbreviated version!
For detailed information about sphingolipid 8-(E/Z)-desaturase, go to the full flat file.
Reaction
Synonyms
8 fatty acid desaturase, 8-sphingolipid desaturase, D8A, D8B, D8C, D8D, DELTA8 Des, Delta8 sphingolipid desaturase, DELTA8(E,Z)-sphingolipid desaturase, Delta8-fatty-acid desaturase 1, Delta8-fatty-acid desaturase 2, DELTA8-sphingolipid desaturase, Delta8-sphingolipid desaturase 1, Delta8-sphingolipid desaturase 2, Delta8-sphingolipid desaturase 3, DELTA8-sphingolipid-desaturase, DES8, DXDES, LCB DELTA8 desaturase, PtD8A, PtD8B, PtD8C, SLD, SLD1, SLD2, sphingoid long-chain base DELTA8 desaturase, sphingolipid DELTA8-desaturase
ECTree
1.14.19.29 sphingolipid 8-(E/Z)-desaturaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.14.19.29 - sphingolipid 8-(E/Z)-desaturase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
fluorinated phytosphinganine + ferrocytochrome b5 + O2 + H+
?
-
the presence of a fluorine atom at the position of the initial hydrogen removal C8-HR leads to a complete suppression of the desaturation reaction, while replacement of C8-HS with fluorine generates a mixture of mainly (Z)- and trace amounts of (E)-fluoroolefine
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
sphinganine + ferrocytochrome b5 + O2 + H+
8-sphingenine + ferricytochrome b5 + H2O
-
-
-
?
sphinganine ceramide + ferrocytochrome b5 + O2 + H+
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
39.8% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
7.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
the enzyme exhibits preference for (Z)-isomer formation
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
(2)
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
(2)
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
no activity with palmitoleic acid, linoleic acid, and alpha-linolenic acid
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine
-
-
?
additional information
?
-
-
isozyme PtD8A preferably forms (8E)-products, ratio 8(Z):8(E) is 0.25
-
-
?
additional information
?
-
-
isozyme PtD8B preferably forms (8E)-products, ratio 8(Z):8(E) is 0.17
-
-
?
additional information
?
-
-
isozyme PtD8C preferably forms (8Z)-products, ratio 8(Z):8(E) is 12.71
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
