1.14.19.26: acyl-[acyl-carrier-protein] 6-desaturase
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For detailed information about acyl-[acyl-carrier-protein] 6-desaturase, go to the full flat file.
Reaction
+ 2 reduced ferredoxin [iron-sulfur] cluster + + 2 H+ = + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Synonyms
DELTA6 16:0-ACP desaturase, DELTA6 desaturase, DELTA6 palmitoyl-ACP desaturase, DELTA6-16:0-ACP desaturase, DELTA6-palmitoyl (16:0)-ACP desaturase, DELTA6-sphingolipid desaturase, DES6
ECTree
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Engineering
Engineering on EC 1.14.19.26 - acyl-[acyl-carrier-protein] 6-desaturase
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A181T/A188G/Y189F/S205N/L206T/G207A
the mutation yields an enzyme that functions primarily as a DELTA9-18:0-[acyl-carrier protein] desaturase. This enzyme displays only DELTA9 desaturase activity with 18:0-[acyl-carrier protein] and is nearly 4-fold more active with this substrate than with 16:0-[acyl-carrier protein]. Like mutant A181TyA200F, this enzyme retains DELTA6 desaturase activity with 16:0-[acyl-carrier protein]
A181T/A200F
the mutation gives rise to an enzyme that catalyzed primarily the DELTA9 desaturation of 18:0-[acyl-carrier protein], but functions as a DELTA6 desaturase with 16:0-[acyl-carrier protein]
A181T/A200F/S205N/L206T/G207A
replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase. The A181T/A200F/S205N/L206T/G207A mutant enzyme is functions principally as a DELTA9-18:0-[acyl-carrier protein] desaturase
A188G/Y189F
mutant with broadened fatty acid chain-length specificity
additional information
replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase, mutant enzymes are identified that have altered fatty acid chain-length specificities or that can insert double bonds into either the DELTA6 or DELTA9 positions of 16:0- and 18:0-[acyl-carrier protein]