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1.14.19.2: stearoyl-[acyl-carrier-protein] 9-desaturase

This is an abbreviated version!
For detailed information about stearoyl-[acyl-carrier-protein] 9-desaturase, go to the full flat file.

Word Map on EC 1.14.19.2

Reaction

stearoyl-[acyl-carrier protein]
+ 2 reduced ferredoxin [iron-sulfur] cluster +
O2
+ 2 H+ =
oleoyl-[acyl-carrier protein]
+ 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O

Synonyms

acyl-ACP-desaturase, acyl-[acyl-carrier-protein] desaturase, ahSAD3A, ahSAD3B, AtSAD1, CrFAB2, CSAD, DELTA9 desaturase, DELTA9-stearoyl-ACP-desaturase-C, DesA1, DesA2, desaturase, acyl-[acyl carrier protein], EC 1.14.99.6, FAB, FAB2, Gmsacpd-c, NbSACPD-A, NbSACPD-B, NbSACPD-C, OeSAD2, OeSAD3, OsSAD1, OsSAD2, OsSAD3, PpSAD, PtSAD, S-ACP-DES, S-ACP-DES1, S-ACP-DES2, S-ACP-DES3, S-ACP-DES4, S-ACP-DES5, S-ACP-DES6, SACPD, SACPD-C, SAD, SAD1, SAD2, SAD3, SAD4, SAD6, SAD7, SAD8, SLL1, SSI2 desaturase, SSI2/FAB2, stearoyl-ACP desaturase, stearoyl-acyl carrier protein (ACP) desaturase, stearoyl-acyl carrier protein desaturase, stearoyl-acyl carrier protein desaturase 3, stearoyl-acyl carrier protein desaturase-1, stearoyl-acyl carrier protein fatty acid desaturase, stearoyl-[acyl carrier protein] desaturase, stearyl acyl carrier protein desaturase, stearyl-ACP desaturase, stearyl-acyl carrier protein desaturase, Tc01g009910, Tc04g005590, Tc04g017510, Tc04g017520, Tc04g017540, Tc05g012840, Tc08g012550, Tc09g024040, TcSAD1, TcSAD2, TcSAD3, TcSAD4, TcSAD5, TcSAD6, TcSAD7, TcSAD8, XsSAD, ZmSAD1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.2 stearoyl-[acyl-carrier-protein] 9-desaturase

Cloned

Cloned on EC 1.14.19.2 - stearoyl-[acyl-carrier-protein] 9-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a Brassica napus bacterial artificial chromosome, BAC, library is constructed
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cloned in pQE-30 vector and expressed in Escherichia coli strain M15, His-tag
cloning of isoform 1
cloning of isoform 2
DesA1 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid
DesA2 overexpressed in Escherichia coli BL21-(DE3) with the natural N-terminal methionine residue as the first amino acid
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression of 2 isoforms in Escherichia coli
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expression of cDNA in Escherichia coli
full-length cDNA clone
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gene CocoFAD, isolated from a cDNA library prepared from the endosperm of coconut, DNA and amino acid sequence determmination and analysis, sequence comparisons and phylogenetic analysis and tree, real-time fluorescent quantitative PCR expresion analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1, the levels of palmitoleic acid (16:1) and oleic acid (18:1) are improved significantly, stearic acid (18:0) is reduced in recombinant cells
gene FAB2, real-time quantitative PCR enzyme expression analysis
gene FAB2, sequence comparisons, semi-quantitative RT-PCR expression analysis, recombinant overexpression in Chlamydomonas reinhardtii
gene Gmsacpd-c, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
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gene NbSACPD-A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene NbSACPD-B, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene NbSACPD-C, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene sad, cloning from embryo, DNA and amino acid sequence determination and analysis, quantitative and semi-quantitative real-time RT-PCR enzyme expression analysis, recombinant XsSAD expression in Escherichia coli cells resulting in increased 18:1D9 level, and confirming the biological activity of the enzyme encoded by XsSAD. Recombinant XsSAD expression in and partial complementation of Arabidopsis thaliana ssi2 mutant, via Agrobacterium strain GV3101 transformation method,control of 35S promoter partially restors the morphological phenotype and effectively increases the 18:1D9 level. The levels of other unsaturated fatty acids synthesized with 18:1D9 as the substrate also increase to some degree
gene SAD, DNA and amino acid sequence determination and analysis, enzyme expressioon analysis
gene SAD, DNA and amino acid sequence determination and analysis, ZmSAD1-based association mapping, genotyping, sequence comparisons, phylogenetic analysis, quantitative reverse transcription PCR enzyme expression analysis
gene sad, multiple copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
gene SAD1, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD1, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD1, phylogenetic analysis
gene SAD1, phylogenetic analysis, ZmSAD1 overexpression (ZmSAD1), antisense ZmSAD1 (anti-ZmSAD1), and ZmSAD1 RNA interference (ZmSAD1 RNAi) constructs are generated in the pBI121 vector under control of the seed-specific FAE1 promoter, recombinant expression in Arabidopsis thaliana with higher expression levels in mature seeds and immature siliques than in roots, stems, leaves, and petals. Fatty acid composition and content are modified in the transgenic Arabidopsis seeds, seed-specific overexpression of the exogenous ZmSAD1 gene significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids, composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs. Phenotypes, overview
gene SAD2, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD2, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD3, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD3, cloning of the isozyme from Olea europaea cv. Picual, DNA and amino acid sequence determination and analysis, isozyme sequence comparison and phylogenetic analysis, expression analysis
gene SAD4, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD5, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD6, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, recombinant expression in Arabidopsis thaliana homozygous fab2 mutant, ssi2 mutant background, driven by CaMV 35S promoter
gene SAD7, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis, SAD7 cannot be recombinantly expressed in Arabidopsis thaliana
gene SAD8, cloned from leaves, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time PCR isozyme expression analysis
into the pUCm-T vector and subcloned into the vector pET30a for expression in Escherichia coli BL21DE3 cells
into the vector pQE-30 for expression in Escherichia coli M15 cells
OeSAD2 is not expressed as a soluble protein
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S-ACP-DES1 expressed in Escherichia coli
S-ACP-DES3 enzyme expressed in Escherichia coli
S-ACP-DES4 enzyme expressed in Escherichia coli
S-ACP-DES5 enzyme expressed in Escherichia coli