Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.19.1: stearoyl-CoA 9-desaturase

This is an abbreviated version!
For detailed information about stearoyl-CoA 9-desaturase, go to the full flat file.

Word Map on EC 1.14.19.1

Reaction

stearoyl-CoA
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
oleoyl-CoA
 + 2 ferricytochrome b5 + 2 H2O

Synonyms

ACOD4, acyl coenzyme A desaturase, acyl-CoA desaturase, delta-9 desaturase, DELTA9 desaturase, DELTA9 fatty acid desaturase, DELTA9 terminal desaturase, DELTA9-acyl CoA desaturase, DELTA9-desaturase, DELTA9-fatty-acid desaturase, DELTA9D, DesA3, desaturase 9, Dpu-DELTA9-KPSE, EC 1.14.99.5, eicosatrienoyl-CoA desaturase, FAD9, fatty acid 9-desaturase, fatty acid DELTA9-desaturase, fatty acid desaturase, fatty acyl CoA DELTA9-desaturase, fatty acyl DELTA9-desaturase, fatty acyl-CoA desaturase, hSCD1, hSCD5, long-chain fatty acid DELTA9-desaturase, membrane-bound stearoyl-CoA DELTA9 desaturase, NPVE desaturase, palmitoyl CoA desaturase, palmitoyl-CoA desaturase, SCD, SCD-1, SCD-2, SCD-3, SCD-4, SCD1, SCD2, SCD3, SCD4, SCD5, stearoyl CoA desaturase, stearoyl coenzyme A desaturase, stearoyl coenzyme-A desaturase 1, stearoyl-ACP DELTA9 desaturase, stearoyl-CoA (DELTA8) desaturase, stearoyl-CoA desaturase, stearoyl-CoA desaturase 1, stearoyl-CoA desaturase 2, stearoyl-CoA desaturase enzyme-1, stearoyl-CoA desaturase-1, stearoyl-CoA desaturase-2, stearoyl-CoA desaturase-3, stearoyl-CoA desaturase-4, stearoyl-CoA desaturase-5, stearoyl-CoA desaturase1, stearoyl-CoA desaturase2, stearoyl-coenzyme A desaturase, stearoyl-coenzyme A desaturase-1, stearyl coenzyme A desaturase, stearyl-CoA desaturase, TkSCD-1, TkSCD-2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.1 stearoyl-CoA 9-desaturase

Cloned

Cloned on EC 1.14.19.1 - stearoyl-CoA 9-desaturase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA fragment corresponding to bp 278-2005 of the sequence was subcloned into a pcDNA3 vector in antisense orientation and stably transfected into SV40-WI38 lung fibroblasts cells, enzyme activity reduced in these stable cell clones, reduced protein level, reduced enzyme expression slows the rate of cell proliferation and suppresses anchorage-independent proliferation, SCD deficiency promotes apoptosis in a ceramide-independent manner
coexpression of acyl carrier protein isoform I gene and holo-acyl carrier protein synthase of Escherichia coli
-
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in HEK-293 cell
expressed in HEK-293 cells
-
expressed in HEK-293A cells
-
expressed in HeLa cells, tagged with myc epitope at the C terminus or HA epitope
-
expressed in L6GLUT4myc myoblasts, localized in endoplasmic reticulum-like subcellular structures, laser scanning microscopy
-
expressed in primary human skeletal muscle cells from obese and lean donors, SCD1 mRNA levels are increased 2.8fold, protein levels are increased 5.7fold in myotubes from obese compared to lean subjects
-
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain ole1
expression in Escherichia coli, transfection of Arabidopsis by Agrobacterium tumefaciens clones
-
expression of DELTA9-3 gene in Saccharomyces cerevisiae
-
four stearoyl-CoA desaturase genes
-
gender- and age-related quantitative expression analysis of SCD in brains in relation to the fatty acid composition, overview
-
gene complements genetic disruption of OLE1 of Saccharomyces cerevisiae
gene rv3229c, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli as c-Myc and/or His-tagged protein, DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA. The native C-terminal sequence of LAA apparently serves as a binding determinant for a mycobacterial protease/degradation system directed at DesA3. Fusion of only the last 12 residues of native DesA3 to the C-terminal of GFP is sufficient to make GFP unstable
-
gene scd, lentiviral-based overexpression and knockdown of SCD in tumor-initiating cells
gene SCD, quantitative real-time PCR expression analysis
gene SCD, semiquantitative and quantitative RT-PCR enzyme expression analysis
gene SCD-1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli srain BL21(DE3)
-
gene SCD-2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli srain BL21(DE3)
-
gene SCD1, phylogenetic tree, sequence comparisons with other isozymes and enzymes from other organisms, overview
gene scd1, quantitative real-time PCR enzyme expression analysis
gene scd1, quantitative real-time PCR expression analysis
gene scd1, real-time PCR expression analysis
gene scd1, recombinant expression of isozyme SCD1 in Saccharomyces cerevisiae monounsaturated fatty acid auxotroph L8-14C, which allows growth in media lacking unsaturated fatty acids. Optimization of enzyme expression method
gene scd3, recombinant expression of wild-type and mutant isozymes SCD3 in Saccharomyces cerevisiae monounsaturated fatty acid auxotroph L8-14C, wild-type exxpression allows growth in media lacking unsaturated fatty acids
gene SCD5, DNA and amino acid sequence determination and analysis, phylogenetic tree, sequence comparisons with other isozymes and enzymes from other organisms, overview
heterologous gene expression in yeast
-
heterologously expressed in Saccharomyces cerevisiae
hSCD1 and GFP-hSCD1 expressed in HEK-293 cells
hSCD5 and GFP-fused hSCD5 expressed in HEK-293 cells
identified of positive clones by screening with hybridization of cDNA of carp with rat DELTA9-desaturase cDNA
-
in vitro translation using a wheat germ cell-free extract in the presence of unilamelar liposomes, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes
isozyme SCD1, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in HEK-293 cell microsomes
isozyme SCD2, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in HEK-293 cell microsomes
isozyme SCD3, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in HEK-293 cell microsomes
isozyme SCD5 liver-specific overexpression in isozyme SCD1 global knockout mutants
overexpressed in INS-1 cell
-
overexpression in CHO cells results in a significant increase in the rate of triglyceride synthesis
SCD1 and SCD2 expression analysis
-
SCD1, realtime-PCR-based quantitative expression analysis
SCD2, realtime-PCR-based quantitative expression analysis